AMMONIAGENESIS IN CULTURED RENAL (LLC-PK1) EPITHELIA

肾LLC-PK1融合培养中氨化作用的研究 上皮细胞系。人们发现，合成氨的生产是严格的 依赖于胞外谷氨酰胺的浓度。LLC-PK1细胞 对代谢性酸中毒的反应。LLC-PK1细胞表现出增加 产氨量和谷氨酰胺消耗量随pH降低的变化 培养基料。为了研究氨化作用与成氨作用的关系 并且已经进行了分离LLC-PK1细胞的糖异生尝试 能够发生糖异生的。

Ammoniagenesis was studied in confluent cultures of the LLC-PK1 renal epithelial cell line. It was found that ammonia production was strictly dependent on the extracellular glutamine concentration. LLC-PK1 cells respond to metabolic acidosis. LLC-PK1 cells exhibited an increase in ammonia production and glutamine consumption with decreasing pH of the culture medium. In order to study the relationship between ammoniagenesis and gluconeogenesis attempts have been undertaken to isolate LLC-PK1 cells capable of gluconeogenesis.