Establishment of a physiological culture system to improve translation in metabolic research

这方面的主要产出将是技术。控制细胞氧化状态的能力，与更生理化的细胞培养基相结合，将为脂肪组织生物学的研究提供强有力的新工具。此外，更深入地了解细胞系如何响应其环境对其他细胞模型具有广泛的影响。该项目将为脂肪细胞模型制定标准，提高细胞，小鼠和人类之间的再现性和翻译。用于研究代谢疾病的改进细胞模型将使学术和工业利益相关者受益。事实上，我们提出的技术是廉价和简单的实施，使它可以访问全球观众，而不仅仅是那些基于资金充足的研究环境。我们将通过出版物和剑桥代谢网络网站传播信息，以提供更详细的方案。研究数据管理（RDM）将遵循可查找、可解释、可互操作和可重用（FAIR）原则。

The major output from this will be technological. The ability to control the oxidative status of cells, combined with a more physiological cell culture media, will provide a powerful new tool for the study of adipose tissue biology. Furthermore, a deeper understanding of how cell lines respond to their environment has broad implications for other cellular models. This project will set standards for adipocyte models, improving reproducibility and translation between cells, mice and humans. Improved cellular models for studying metabolic diseases will benefit academic and industrial stakeholders. The fact our proposed technology is cheap and straightforward to implement makes it accessible to a global audience, not just those based in well-funded research environments. We will disseminate information through publication and also the Cambridge Metabolic Network Website to provide more detailed protocols. Research data management (RDM) will follow Findable, Accessible, Interoperable, and Re-usable (FAIR) principles.