CANCER CARIES, LIPID, CYSTIC FIBROSIS AND STRESS RESEARC

拟开展的工作包括：1)铂-嘧啶类化合物的效应 对有丝分裂和酶活性的影响。对影响的调查 瑞士白铂-胸腺嘧啶和铂-尿嘧啶对正常和癌细胞的作用 不同剂量水平和不同时间间隔的小鼠；药物对小鼠的影响 分裂正常细胞和癌细胞的染色体和纺锤体纤维； 药物对大鼠肾细胞碱性和酸性磷酸酶活力的抑制作用 正常细胞和癌细胞；药物对正常细胞和癌症的毒性作用 肠道和肾脏的细胞。2)胰酶及其底物。 不同组分脂解活性的层析和组织化学研究 摄食、禁食和匹罗卡品刺激下的脊椎动物胰腺和十二指肠 条件。3)囊性纤维化患者尿液中的代谢物。分离方式 高速液-液色谱与气相鉴别 色质联用、碳水化合物和氨基酸分析 囊性纤维化患者和健康人尿液中的代谢物。4) 压力相关变量的检测和控制。确定了 脉搏波检测与自主行为控制的关系 速度、外周血流量(热)、阿尔法脑电波频率和 皮肤电位发生变化。

The proposed work includes the following projects: 1) Effect of Pt-Pyrimidines on Mitotic and Enzyme Activity. Investigation of the effects of platinum-thymine and platinum-uracil on normal and cancer cells in Swiss white mice at different dose levels and time intervals; the effects of the drugs on chromosomes and spindle fibers of dividing normal and cancer cells; the inhibitory action of the drugs on alkaline and acid phosphatase activity in normal and cancer cells; and toxic effects of the drugs on normal and cancer cells of the intestine and kidney. 2) Pancreatic enzymes and their substrates. A chromatographic and histochemical study of the lipolytic activity of various vertebrate pancreas and duodenum under fed, fasted, and pilocarpine stimulated conditions. 3) Metabolites in urine of cystic fibrosis patients. Separation by high speed liquid-liquid chromatography and identification by gas chromatography/mass spectrometry, carbohydrate and amino acid analyses of unique metabolites in urine of cystic fibrosis patients and healthy humans. 4) Detection versus control of stress related variables. Determination of the relationship between detection and voluntary behavioral control of pulse wave velocity, peripheral blood flow (thermal), alpha brain wave frequencies, and skin potential changes.