BINDING CHARACTERIZATION AND PROTECTIVE IMMUNITY OF MABS TO TYPE 9V CONJUGATE

建立了抗肺炎链球菌9V多糖(PS)的单抗 采用PS与灭活肺溶血素免疫原偶联而成。 9V型PS单抗中含有IgG1亚类抗体。9V PS MAB 识别9PS组免疫表位并与其反应 与兔9V抗血清中观察到的模式不同 (AS)。以速率法测定抗原抗体反应率的变化 第9组PS-9V单抗反应在低浓度时比浊度迅速增加 浓度，并在10ug ps/ml时达到平台期。相比之下， 第9组PS-兔9V反应速率随反应增加的变化 在20微克的浓度下按比例并继续增加 Ps/ml。 在免疫扩散中，9V单抗与9N PS反应，而不与9N PS反应 兔9V-AS与9N-PS的沉淀反应。此外，9V PS单抗既有光学作用，又能抵御9型病毒的挑战 幼鼠体内的肺炎链球菌。这些结果表明，被动型 免疫与主动免疫相结合可诱导有效 预防青少年肺炎球菌感染的保护性免疫 孩子们。

Monoclonal antibody (MAb) to pneumococcal type 9V polysaccharide (PS) was produced using PS conjugated with inactivated pneumolysin immunogen. Type 9V PS MAb contained subclass IgG1 antibody. The 9V PS MAb recognized and reacted with an immunologic epitope of group 9 PS in patterns which are different from that observed in rabbit 9V antiserum (AS). The change of antigen-antibody reaction rate as measured by rate nephelometry in the group 9 PS-9V MAb reaction increased rapidly at low concentrations and reached a plateau at 10 ug PS/ml. In contrast, the change of reaction rate in group 9 PS-rabbit 9V AS reaction increased proportionally and continued to increase at the concentration of 20 ug PS/ml. In immunodiffusion, 9V MAb reacted with 9N PS whereas there was no precipitin reaction between rabbit 9V AS and 9N PS. Furthermore,the 9V PS MAb is both opsonic and protective against challenge with type 9 pneumococci in young mice. These results indicate that the passive immunity combined with active immunization could induce effective protective immunity for prevention of pneumococcal infection in young children.