CROSS-REACTIVITY OF PNEUMOCOCCAL SURFACE PROTEIN A MAB WITH VACCINE STRAINS

肺炎球菌表面蛋白A(PSPA)是一种免疫优势和保护性蛋白 肺炎链球菌表面蛋白抗原。PSPA的截断版本 已被克隆并在沙门氏菌疫苗中高效表达 其中编码腺苷环化酶和cAMP受体的基因 蛋白质已被删除。经口免疫或免疫的小鼠 重组沙门氏菌-PSPA疫苗滴鼻刺激小鼠 体液和粘膜免疫系统诱导高滴度抗PSPA 儿童免疫球蛋白Ig G、Ig M、Ig A抗体及血清Ig A水平 粘膜分泌物。重组沙门氏菌-PSPA疫苗诱导 口服免疫小鼠的免疫保护性反应及其意义 观察到对毒力强病毒的挑战的保护水平 肺炎链球菌2型，WU2。 几种肺炎球菌疫苗类型的细菌细胞，包括1，9V， 对12F、20、23F和33F进行交叉反应抗原检测 PSPA组织化学酶和荧光抗体染色方法 单抗制剂。对所有类型的阳性反应都是 用重组PSPA抗原的单抗观察 小鼠肺对1型、9V型、19F型和20型有部分反应 洗涤样品。小鼠阴道分泌物未显示免疫性 对这些菌株的反应。

Pneumococcal surface protein A (PspA) is an immunodominant and protective surface protein antigen of S. pneumoniae. A truncated version of PspA has been cloned and expressed at a high level in a Salmonella vaccine strain, in which genes encoding for adenylate cyclase and CAMP receptor protein have been deleted. Mice immunized through oral route or intranasally with the recombinant Salmonella-PspA vaccine stimulated the humoral and mucosal immune systems inducing high serum titers anti-PspA antibodies of IgG, IgM and IgA types and significant levels of IgA in Mucosal secretions. The recombinant Salmonella-PspA vaccine induced immunoprotective responses in orally vaccinated mice and significant levels of protection were observed against challenge with the virulent pneumococcal type 2, WU2. Bacterial cells of several pneumococcal vaccine types including 1, 9V, 12F, 20, 23F and 33F were tested for cross-reactive antigen by histochemical enzyme and fluorescent antibody staining methods using PspA monoclonal antibody preparations. Positive reactions to all types were observed with monoclonal antibodies to recombinant PspA antigen whereas partial responses to types 1, 9V, 19F and 20 were found in mouse lung washing samples. Mouse vaginal secretions did not show immunologic reactions with these strains.