MORPHOLOGIC, IMMUNOCYTOCHEMICAL AND FISH ANALYSES IN CYTOLOGY

在过去的一年里，我的工作重点是形态和 细胞学几个重点领域的免疫细胞化学分析。 正在进行的项目包括：评估海绵体的形态谱 PAP涂片中的萎缩以确定真正的癌前病变和 描述特定的细胞学模式。在试图识别和识别 定量间皮瘤细胞系中IGF-1受体的存在， 我们用一种已知的对照方法测定了IGF-1受体的抗体。 细胞自旋和石蜡包埋材料的不同固定剂。我们 结论：1：5的细胞旋体染色效果最好。 甲醛固定。福尔马林固定石蜡包埋的研究 细胞块切片显示1：10最佳染色。9例间皮瘤 检测细胞系中IGF-1的存在和表达强度 受体呈阳性染色，染色强度从1+到 3+。在Oncor医学主任Sandra Wolman博士的合作下， Inc.，我们处理了25个细胞学样本，试图定义和 鱼体细胞标本最佳加工方法概述 悬液，适用于所有新鲜细胞学标本的技术 也可用于外科病理处理。 吸入物和其他物质。我们得出的结论是所有的样品都应该是 及时处理以确保标本的生存能力并对其进行分类 以个体为基础，确保制备适度细胞 单层胞浆。获得了等效的核探测信号 有几种固定样品的方法：风干，95%乙醇， 甲醇(Diff-Quik固定剂)和Carnoy‘s溶液。没有区别 注意到在核探测器信号或标本粘连上 带正电或不带电的幻灯片。在最初的固定之后，我们的 幻灯片保持在室温下，直到鱼在没有 是否有任何不良影响。用蛋白水解酶K和 随后的重新杂交在样本上产生了阳性结果 最初产生的核探测信号很差。

In the last year, the focus of my work has been on morphologic and immunocytochemical analyses of several focused areas of cytology. Ongoing projects include: evaluation of the morphologic spectrum of atrophy in PAP smears to identify true preneoplastic changes and describe specific cytologic patterns. In an attempt to identify and quantify the presence of IGF - 1 receptors in mesothelioma cell lines, we titered antibody to IGF - 1 receptor using a known control with different fixatives for cytospin and paraffin embedded material. We concluded that cytospins showed optimal staining at 1:5 with formaldehyde fixation. Studies of the formalin-fixed paraffin embedded cell block section showed optimal staining at 1:10. Nine mesothelioma cell lines examined for the presence and intensity of the IGF - 1 receptor revealed positive staining with intensity ranging from 1+ to 3+. In cooperation with Dr. Sandra Wolman, Medical Director of Oncor, Inc., we processed 25 cytology samples in an attempt to define and outline a method for optimal specimen processing for FISH use in cell suspensions, techniques applicable to all fresh cytology specimens which can also be used for the processing of surgical pathology aspirates and other material. We concluded all samples should be promptly processed to ensure specimen viability and triaged on an individual basis to ensure preparation of moderately cellular monolayered cytospins. Equivalent nuclear probe signals were obtained with several sample fixation methods: air-drying, 95% ethanol, methanol (Diff-Quik fixative) and Carnoy's solution. No difference was noted in the nuclear probe signals or specimen adhesion on positively charged or non-charged slides. After initial fixation, our slides remained at room temperature until FISH was performed without any adverse effects. A short digestion with proteinase K and subsequent rehybridization yielded positive results on samples that originally yielded poor nuclear probe signals.