MEGAKARYOCYTE-SPECIFIC GENES & GENES ASSOCIATED/W CLONAL BONE MARROW DISORDERS

我们正在继续这个始于1993年的项目。我们一直致力 建立人巨核细胞的培养和富集方法。 直到1995年8月，我们一直在收集外周血， 普通志愿者我们现在已经有了NHLBI IRB批准的方案 用于采集正常志愿者的骨髓标本， 我们希望巨核细胞祖细胞会更丰富。一旦我们 建立了足够的富集培养物，来源于正常 个体，我们将开始收集骨髓样本， 来自克隆性血液病患者的外周血。我们将 通过各种技术比较来自两个来源的消息。

We are continuing this project, which began in 1993. We have been working to establish culture and enrichment procedures for human megakaryocytes. Until 8/95, we were working with collections of peripheral blood from normal volunteers. We have now had a protocol approved by the NHLBI IRB for the collection of bone marrow specimens from normal volunteers, which we expect to be richer in megakaryocyte progenitors. Once we have established sufficient enrichment in cultures derived from normal individuals, we will begin collecting samples of bone marrow and peripheral blood from patients with clonal hematologic disorders. We will compare messages from both sources by a variety of techniques.