DETECTING MEGAKARYOCYTES AND GENES ASSOCIATED WITH CLONAL BONE MARROW DISORDER

这个正在进行的项目包括几个阶段。我们有一个协议 采集正常志愿者的骨髓用于 培养相对纯净的巨核细胞群体。我们当时在 巨核细胞培养条件的建立过程 丰富，但商业培养试剂盒最近可用 为了这个目的。在过去的一年里，我们一直在开发一种 具有代表性的体细胞DNA突变检测方法 差异分析。当此检测方法可靠地与测试一起工作时 样本，我们将开始分析患者样本。

This ongoing project involves several stages. We have a protocol for collecting of bone marrow from normal volunteers to use for the culture of relatively pure populations of megakaryocytes. We were in the process of developing the culture conditions for megakaryocyte enrichment, but commercial culture kits recently became available for this purpose. Over the past year, we have been developing an assay for detecting mutations in somatic DNA using representational difference analysis. When this assay works reliably with test samples, we will begin to analyze patient specimens.