DETECTING MEGAKARYOCYTES AND GENES ASSOCIATED WITH CLONAL BONE MARROW DISORDER

检测与克隆性骨髓疾病相关的巨核细胞和基因

• 批准号: 2571431
• 负责人: S ROSENFELD
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2571431
• 关键词: bone marrow disorder; gene mutation; human genetic material tag; human tissue; megakaryocytes; tissue /cell culture

This ongoing project involves several stages. We have a protocol for collecting of bone marrow from normal volunteers to use for the culture of relatively pure populations of megakaryocytes. We were in the process of developing the culture conditions for megakaryocyte enrichment, but commercial culture kits recently became available for this purpose. Over the past year, we have been developing an assay for detecting mutations in somatic DNA using representational difference analysis. When this assay works reliably with test samples, we will begin to analyze patient specimens.

这一正在进行的项目包括几个阶段。我们有一个协议， 从正常志愿者中收集骨髓用于 培养相对纯的巨核细胞群体。我们在 巨核细胞培养条件的开发过程 但最近商业化的培养试剂盒已经上市， 用于此目的。在过去的一年里，我们一直在开发一个 使用代表性DNA检测体细胞DNA中突变的测定法 差异分析当该检测试剂盒与检测试剂盒可靠地配合使用时， 样本，我们将开始分析患者样本。