IDENTIFICATION OF DRUG RELATED GENES

滥用药物通过生化作用导致行为的长期变化 这些机制在很大程度上是未知的。药物改变后的表达变化 大脑中的特定基因可以提供一个主要的窗口 成瘾的生化底物。为了进一步探索这一领域，我们 建立和发展了减法差分显示 技术已经允许识别200多个不同的 与候选药物调控基因相对应的cDNA在 大脑。在本财年，我们已经确定了20个候选基因的特征 表达受安非他明、可卡因或吗啡的调节。数列 对这些cDNA的分析表明，它们分为三类：新奇的 CDNA，以前仅描述为序列的那些，以及那些落在 具有功能含义的特定基因家族

Abused drugs produce long-lasting changes in behaviors via biochemical mechanisms that are largely unknown. Drug-altered changes in expression of specific genes in the brain can provide a major window on possible biochemical substrates for addiction. To further explore this area, we have established and developed subtracted differential display techniques that have allowed identification of more than 200 distinct cDNAs that correspond to candidate drug-regulated genes expressed in brain. During this FY, we have characterized 20 of candidate genes whose expression is regulated by amphetamine, cocaine or morphine. Sequence analyses of these cDNAs reveal that they fall into three groups: novel cDNAs, those previously described as sequences only, and those that fall into specific gene families with functional implications