NOVEL GENES WITH ALTERED EXPRESSION DURING DRUG TREATMENT AND WITHDRAWAL

基因表达的变化可以在有机体的整个生命周期中保持不变 并可能是成瘾行为长期变化的基础。被虐待 药物-导致长期的行为障碍，被认为是 由中枢神经系统中的生物机制维持 在很大程度上是未知的。在前几个财年期间在此组中的工作 开发了识别此类基因的方法和有记录的候选基因 表达受滥用药物调控的基因，包括 苯丙胺、可卡因和吗啡，但代表没有 基因库搜索中的同源基因。这类新基因中的一个cdna是 被追捕。大鼠大脑皮质2.4kb文库的筛选 在大鼠大脑皮层识别8kb信使核糖核酸的基因。进一步 研究将评估这种基因产物的大脑分布，其 与开发和额外药物治疗有关的监管，以及其他 现在看来代表一个原型成员的基因同源 新奇的药物调控基因家族。

Changes in gene expression can be maintained over the life of an organism and may underlie long term changes in addictive behavior. Abused drugs-induce long-term behavioral disorders that are thought to be maintained by biological mechanisms in the central nervous system that are largely unknown. Work in this group during previous FYs has developed approaches to identifying such genes and documented candidate genes whose expression are regulated by abused drugs, including amphetamine, cocaine and morphine, but represent novel sequences without homologs in genebank searches. One cDNA in this class of new genes was pursued. Screening of rat cortex cDNA libraries produced a 2.4 kb in cDNA that recognizes to a 8 kb mRNA in rat cerebral cortex. Further studies will assess the brain distribution of this gene product, its regulation with development and additional drug treatments, and other gene homologs of what now appears to represent a prototypic member of a novel, drug-regulated gene family.