FLASH PHOTOLYSIS OF MICROSOMAL P450

研制了一台激光闪光光解仪，用于测量材料的表面张力。 一氧化碳与肝脏P450细胞色素结合的动力学研究 用各种药物和致癌物处理的大鼠的微粒体。 由于多种形式的P450在整个反应中起作用，因此 采用差分动力学方法对动力学行为进行判别 个人的P450。这种方法需要分析差异。 在没有和存在特定分子的情况下的动力学分布之间 P450效应器，并成功地获得了 单个P450参与药物和致癌物代谢。 具体地说，不同的多环烃 加速CO与P450 1A1的结合，从而代谢这些 致癌物以大小和形状依赖的方式。除了……之外 大鼠肝脏微粒体，这种方法被应用于单个人 P450。对人P450 3A4进行了评价，不同底物 以底物依赖的方式调节CO结合动力学。

A laser flash-photolysis apparatus was developed to measure the kinetics of carbon monoxide (CO) binding to P450 cytochromes in liver microsomes from rats treated with various drugs and carcinogens. Since numerous forms of P450 contribute to the overall reaction, a difference kinetic method was used to distinguish the kinetic behavior of individual P450s. This method entails analysis of the difference between the kinetic profiles in the absence and presence of a specific P450 effector, and successfully yielded kinetic parameters for individual P450s involved in drug and carcinogen metabolism. Specifically, various polycyclic hydrocarbons differentially accelerated CO binding to the P450 1A1 form, which metabolizes these carcinogens in a size- and shape-dependent manner. In addition to rat- liver microsomes, this approach was applied to a single human P450. Human P450 3A4 was evaluated, and different substrates modulated the CO binding kinetics in a substrate-dependent manner.