Developing high throughput single cell proteomics

揭示组织或细胞群体中蛋白质表达的变异性对于理解细胞如何响应其环境至关重要。例如，这可以提供关于细胞对感染或化疗的反应的细节。到今天为止，单细胞蛋白质分析已经变得触手可及，但其应用仍然繁琐，并且其通量低。该提案旨在开发和部署一种简单而强大的方法来分析单细胞中的蛋白质含量。

Unraveling the variability in the protein expression in tissues or cell populations is critical to understand how cells respond to their environment. For example, this can provide details about the cellular response to an infection or to a chemoterapic. As of today, single cell protein analyses has become at reach, but its application is still cumbersome and its throughput is low. This proposal aims to develop and deploy a simple and robust method to analyse the protein content in single cells.