BURST GENERATION AND GAMMA BAND OSCILLATIONS

多年来，人们已经知道，不同的唤醒状态可以根据脑电图中观察到的神经振荡的主要频率进行分类。 伽马波段振荡（30- 70 Hz）的出现是从睡眠状态过渡到唤醒状态的标志。 它提出了一个子类的颗粒上锥体细胞，震颤细胞，放电高频动作电位（大于250赫兹）与爆发间的间隔在γ-波段范围内是重要的新皮层γ-波段振荡的产生。 初步的体外研究表明，通过注入去极化电流，一类锥体神经元可以被诱导产生比颤振细胞更强的动作电位。 将通过爆发产生神经元的细胞内记录和猫初级视觉皮层颗粒上层的局部场电位监测，在体内评估网状结构激活中心电刺激后γ-带振荡的发作。 体外去极化电流和胆碱能激动剂的应用将被用来研究爆发式放电的起源和生物物理变化，神经元经历时，它的开关从单一的动作电位放电动作电位的爆发。 通过采用在体内和体外记录方法的作用，爆发产生神经元在神经元同步化和伽玛带振荡的发展将评估。

For many years it has been known that different states of arousal can be classified based upon the predominant frequency of neural oscillations observed in electroencephalograms. The emergence of gamma-band oscillations (30-70Hz) is a hallmark of the transition from sleep states to states of arousal. It is proposed that a subclass of supragranular pyramidal cells, chattering cells, which discharge high frequency action potentials (greater than 250Hz) with interburst intervals in the gamma-band range are important in the generation of neocortical gamma-band oscillations. Preliminary in vitro work has shown that a sub- class of pyramidal neurons can be induced to discharge of action potentials that chattering cells by injection of depolarizing current. The onset of gamma-band oscillations following electrical stimulation of reticular formation activating centers will be assessed in vivo, via intracellular recordings from burst generating neurons and the monitoring of local field potentials in the supragranular layers of cat primary visual cortex. In vitro depolarizing current and the application of cholinergic agonists will be used to study the genesis of burst firing and the biophysical changes that the neuron undergoes when it switches form firing single action potentials to bursts of action potentials. By employing both in vivo and in vitro recording methodologies the role that burst generating neurons have in the development of neuronal synchronization and gamma-band oscillations will assessed.