CORE--IMAGING/BIOMEDICAL ENGINEERING CORE

Imaging Core使用各种可视化技术， 从生物材料中提取形态和生理信息。 核心区由6个设施组成，每个设施提供专门的 服务 1.通过组织学分析，研究人员获得了组织 固定、切片并染色用于光学显微镜检查。 2.超微结构 FACTORY处理组织，用于透射和/或扫描电子 显微镜 3.原位杂交因子处理组织， 使用原位杂交确定mRNA的细胞分布 技术. 4.视频显微镜工厂拥有3个研究级 为一般观察而专门配备的显微镜，用于定量 荧光和各种其他测量。 5.显微荧光法 FACEBOOK具有连接到荧光分光光度计的显微镜， 允许研究人员以更高的增益和时间测量荧光 分辨率比视频显微镜可用。 6.共焦 显微镜FACTORY允许用户查看他们的细胞与共聚焦 显微镜用于精确的Z轴定位荧光标记的 proteins. 成像核心还具有数字图像处理功能 工作站允许调查人员以各种方式量化图像， 输出这些图像的高质量打印以用于演示目的。

The Imaging Core uses a wide variety of visualization techniques to extract morphologic and physiologic information from biological materials. The Core is structured at 6 facilities, each offering specialized services. 1. Through the HISTOLOGY FACILITY investigators have tissues fixed, sectioned, and stained for light microscopy. 2. The ULTRASTRUCTURE FACILITY processes tissues for transmission and/or scanning electron microscopy. 3. The IN SITU HYBRIDIZATION FACILITY processes tissues to determine the cellular distribution of mRNA using in situ hybridization techniques. 4. The VIDEO MICROSCOPY FACILITY houses 3 research-grade microscopes specially equipped for general viewing, for quantitation of fluorescence, and for various other measurements. 5. The MICROFLUORIMETRY FACILITY possesses a microscope connected to a spectrofluorimeter which allows investigators to measure fluorescence at higher gain and temporal resolution than is available by video microscopy. 6. The CONFOCAL MICROSCOPE FACILITY allows users to view their cells with confocal microscopy for precise Z-axis localization of fluorescent-labeled proteins. The Imaging Core also possesses digital image processing workstations allowing investigators to quantify images in various ways to output high-quality prints of these images for presentation purposes.