RESPIRATORY AFFERENTS AND CENTRAL NERVOUS SYSTEM INTERACTIONS

该项目的长期目标是深入了解如何 来自呼吸传入的信息被整合在 中枢神经系统，用于调节呼吸。实验 计划中的计划代表着我们在 该计划的第一年，重点是横隔膜传入的能力 来调节呼吸马达的输出。需要检验的假设 在目前的应用是小脑(顶核在 特别是)作为S治疗膈肌的重要中枢整合部位 以及其他呼吸传入在反射调制中的作用 呼吸量。正在进行的研究利用麻醉的， 去小脑制剂相当令人信服地证明了 小脑参与了两者所引起的全身反射反应。 机械和化学呼吸挑战。额外的实验 计划：评估小脑及其深部的受累情况 核团对激活特异性神经元的反射反应的调制作用 呼吸输入(迷走神经、膈、肋间、腹部、颈动脉 身体和中枢化学感受器)；确定激活的效果 特定小脑内的胞体和/或通路纤维 核团对中枢性呼吸相关神经元放电行为的影响 并将单位射击行为与随之而来的 膈运动输出；研究脑桥呼吸的作用 群和Botzinger复合体在小脑调制中的作用 膈运动输出；以及，表征个体的反应 与呼吸相关的小脑神经元对电、机械和 呼吸输入的化学激活。结果应该有助于 根据我们对常压和低氧时呼吸调节的理解 在正常和病理情况下呼吸紧张。

The long term objectives of this project is to gain insight into how information emanating from respiratory afferents is integrated in the central nervous system and used to modulate respiration. Experiments planned represent a natural progression from our original proposal in year 1 of the program which focused on the ability of diaphragm afferents to modulate the respiratory motor output. the hypothesis to be tested in the present application is that the cerebellum (fastigial nucleus in particular) serves as s significant central integrative site for phrenic as well as other respiratory afferents in the reflex modulation of respiratory output. Ongoing studies utilizing the anesthetized, decerebellate preparation have demonstrated rather convincingly that the cerebellum contributes to the general reflex responses elicited by both mechanical and chemical respiratory challenges. Additional experiments are planned to: evaluate the involvement of the cerebellum and its deep nuclei in the modulation of reflex responses to activation of specific respiratory inputs (vagus, phrenic, intercostal, abdominal, carotid bodies, and central chemoreceptors); determine the effect of activating either cell bodies and/or fibers of passage within particular cerebellar nuclei on the firing behavior of meduallary respiratory-related neurons and correlate unit firing behavior with concomitant changes in the phrenic motor output; investigate the role of the pontine respiratory group and the Botzinger Complex in the cerebellar modulation of the phrenic motor output; and, characterize the response of individual respiratory-related cerebellar neurons to electrical, mechanical and chemical activation of respiratory inputs. The results should contribute to our understanding of respiratory regulation during eupneic and stressed breathing in normal and pathological conditions.