RHEOLOGICAL DETERMINANTS OF MICROVASCULAR FUNCTION

这项研究的总体目标是阐明血液的作用 流变学作为健康和健康人群微血管功能的决定因素 疾病。为此，活体显微镜技术将被 用于评价红细胞(RBC)的变化程度 变形性和聚集性以及白细胞(WBC)变形性 和内皮细胞的黏附，影响血流阻力 血管范围从真正的毛细血管，到小动脉和小静脉 为他们服务。微血管功能的量化指标将 由直接原位测量血流动力学变量得出 外部化组织，如肠系膜、大网膜和提睾肌 肌肉。具体目的是阐明两者之间的关系 个体内血液流动和血液结肠机械特性 微血管，在整个微血管网络的分支点和 在整个区域内，主要的微血管分支都是连续的。 这项研究的主要重点将是阐明微血管 在低流量状态下通过以下两种方式起作用 动脉流入、出血性低血压或药理学 干预。对红细胞的具体研究将描述红色的作用 白细胞边集中的细胞聚集，区域抗性 血流和从长时间的缺血发作中恢复。研究：关于 红细胞变形性将寻求阐明其降低的影响 关于组织缺氧过程中的毛细血管募集过程， 它对区域水流阻力和阻力的影响 在单个未分枝的微血管中流动。关于红细胞浓度的研究 将致力于阐明平均组织之间的关系 红细胞压积，由红细胞流的动力学决定 微血管系统和试管红细胞压积，通过直接获得 在单个微血管中进行测量。白细胞变形性的作用 将被检测为白细胞与内皮细胞黏附的决定因素 流动阻力。光学切片显微镜技术将成为 应用于阐明微血管中的不规则现象的作用 管腔在影响血流阻力方面发挥作用。这是意料之中的 这些研究的结果将为我们深入了解 影响血细胞力学和生化特性的因素 微血管功能和辅助开发治疗策略 治疗各种病理疾病，如贫血、红细胞增多症、低血压病 血流状态、休克、炎症和血细胞紊乱，仅举几例。

The overall goal of this research is to elucidate the role of blood rheology as a determinant of microvascular function in health and disease. To this end, techniques of intravital microscopy will be applied to evaluate the extent to which red blood cell (RBC) deformability and aggregation, and white blood cell (WBC) deformability and adhesion to the endothelium, affect the resistance to blood flow in vessels ranging from the true capillaries, to the arterioles and venules which serve them. Quantitative indices of microvascular function will be derived from direct in situ measurements of hemodynamic variables in exteriorized tissues such as the mesentery, omentum and cremaster muscle. Specific aims are to elucidate the relationship between blood flow and blood coli mechanical properties within individual microvessels, at branch points throughout the microvascular network and regionally throughout the succession of major microvascular divisions. A major emphasis of the research will be to elucidate microvascular function in the low flow state by either mechanical obstruction of the arterial inflow, hemorrhagic hypotension or pharmacological intervention. Specific studies on RBCs will delineate the role of red cell aggregation in the margination of WBCs, regional resistance to blood flow and recovery from prolonged ischemic episodes. Studies on RBC deformability will seek to elucidate the effect of its decrease on the process of capillary recruitment during tissue hypoxia, its effect on regional resistance to flow and on the resistance to flow in single unbranched microvessels. Studies on RBC concentration will aim to elucidate the relationship between average tissue hematocrit, determined by the dynamics of red cell flux through the microvasculature, and the tube hematocrit, obtained by direct measurements in individual microvessels. The role of WBC deformability will be examined as a determinant of WBC adhesion to the endothelium and resistance to flow. Techniques of optical sectioning microscopy will be applied to elucidate the role that irregularities in the microvessel lumen play in affecting the resistance to blood flow. It is anticipated that the results of these studies will provide insight into the role of mechanical and biochemical properties of blood cells in affecting microvascular function and aid development of therapeutic strategies to treat a variety of pathologies such as anemia, polycythemia, the low flow state, shock, inflammation and blood cell disorders, to name a few.