WOUND HEALING IN THE CORNEAL STROMA

1.组织培养基将被改装，试图引起 组织培养的角膜内皮细胞单层的获得 由它们的电学特性指示的正常功能状态。 2.将改装灌注液以维护角膜 处于良好状态的灌流的孤立眼 有可能。组织的行为将与 一种用同样的介质灌流的离体角膜，以便 测试睫状体分泌的一种成分是否是必需的 为了它的福利。 3.损伤后不同细胞类型的动态变化 为了弄清它们的来源，将跟踪角膜基质， 转变和命运。 4.孤立性角膜损伤后的细胞反应 充血的眼睛，在基质的范围内，在粘合的下面 将对隐形眼镜进行检查，以确定 血液和泪水对细胞和刺激的贡献。 5.将悬浮在生理介质中的脂质体用作 流体流过内皮表面的指示物，以便 确定液压泵是在 细胞。 6.钠、钾、氯和钠的电化学电势差 穿过内皮的HCO3将通过带来离子- 靠近细胞层的敏感电极和阻挡离子通量。 一个潜在步骤的发展将建立一个离子是 参与了一个积极的运输机制。

1. Tissue culture media will be modified in an attempt to cause tissue cultured corneal endothelial monolayers to acquire their normal functional state as indicated by their electrical properties. 2. Perfusion media will be modified so as to maintain the cornea of a perfused isolated eye in good condition for as long as possible. The behavior of the tissue will be compared with that of an isolated cornea perfused with the same medium, in order to test whether a component secreted by the ciliary body is essential to its welfare. 3. The kinetics of the different cell types within an injured corneal stroma will be followed in order to clarify their origins, transformations and fate. 4. The cellular reactions to corneal injuries in the isolated perfused eye, in the confines of the stroma, and under a glued-on on contact lens will be examined, with a view to identifying the cellular and stimulatory contributions from the blood and tears. 5. Liposomes suspended in physiological medium will be used as indicators of fluid flow across the endothelial surface in order to determine whether the fluid pump operates between or across the cells. 6. An electrochemical potential difference of Na, K, Cl and HCO3 across the endothelium will be looked for by bringing ion- sensitive electrodes close to the cell layer and blocking ion fluxes. The development of a potential step will establish that an ion is involved in an active transport mechanism.