CELL ADHESION AND MATRIX IN VERTEBRATE DEVELOPMENT

拟议研究计划的主要目标是建立 整合素和ECM分子对细胞增殖的功能性贡献 帮助形成早期两栖动物的形态发生过程 胚胎 为了更好地了解整合素的功能和表达 在胚胎发生中，非洲爪蟾整联蛋白α亚基特异性cDNA和 将获得抗体。 免疫细胞化学和原位 将利用杂交方法以确定空间位置。 以及胚胎中整合素mRNA和蛋白质表达的时间模式。 然后将采用几种方法来分析 特异性整合素受体。 整合素转录本将被注入到 卵子和特定的卵裂球和粘附，迁移和命运的 随着发育进程而确定的后代。 粘合剂和迁移剂 胚胎细胞过度表达控制和显性的行为， 将比较阴性整联蛋白构建体。 整合素的变化 介导的细胞粘附反应诱导将研究使用 来自注射了转录物的胚胎的细胞和外植体 编码已知诱导中胚层的肽。 整合素的调控 整合素配体结合活性的表达和/或活化 激活素诱导的组织也将在mRNA和蛋白质水平进行研究。 程度.

The primary goal of the proposed research program is to establish the functional contribution of integrins and ECM molecules to the morphogenetic processes that help shape the early the early amphibian embryo. In order to better understand integrin function and expression in embryogenesis, Xenopus integrin alpha subunit specific cDNAs and antibodies will be obtained. Immunocytochemistry and in situ hybridization methods will be utilized in order to determine the spatial and temporal patterns of integrin mRNA and protein expression in embryos. Several approaches will then be employed to analyze the functions of specific integrin receptors. Integrin transcripts will be injected into eggs and specific blastomeres and the adhesion, migration and fate of the progeny determined as development proceeds. The adhesive and migratory behaviors of embryonic cells over-expressing control and dominant- negative integrin constructs will be compared. Changes in integrin mediated cell adhesion in response to induction will be studied using cells and explants derived from embryos injected with transcripts encoding peptides known to induce mesoderm. The control of integrin expression and/or the activation of integrin ligand binding activity in activin-induced tissues will also be investigated at the mRNA and protein levels.