ELECTRON TRANSFER WITHIN PROTEIN COMPLEXES

描述：(一字不差地摘自申请者的摘要)远距离研究 ET在现代化学中的核心作用是通过发展 (I)连接的供体-受体模型化合物；(Ii)氧化还原修饰的蛋白质；以及， 我们研究的重点是(Iii)修饰的蛋白质-蛋白质复合体。我们的 该计划解决了两个基本的和互补的问题 蛋白质间电子转移(ET)的关键过程 三个实验系统的结构和动力学特征是什么 控制ET合作伙伴之间的接口识别和对接？何谓 控制实际情况的结构、动力和能量特征 蛋白质-蛋白质复合体内的电子转移事件？混合金属 血红蛋白(Hb)杂交体为研究ET事件提供了无与伦比的机会 在预对接的复合体内，跨越结晶学定义的， 可实验操作的蛋白质-蛋白质(亚基间)界面。 细胞色素C过氧化物酶(CCP)是一种具有多个， 与其ET相互作用的氧化还原中心和多个结合域 伴侣，细胞色素c(CC)。具有重要生理意义的内毒素复合体 细胞色素b5(B5)与肌红蛋白(Mb)和Hb存在互补问题： [mb，b5]低分子量伙伴体系提出了以下基本问题 界面识别和对接；[hb，b5]对提供了机会 探讨Et与a的四元组装和构象的偶联 多亚基蛋白质。

DESCRIPTION: (Verbatim from the Applicant's Abstract) The study of long-range ET has attained a central role in modern chemistry through the development of (i) linked donor-acceptor model compounds; (ii) redox-modified proteins; and, the focus of our research, (iii) modified protein-protein complexes. Our program addresses the two fundamental and complementary questions underlying the critical process of inter-protein electron-transfer (ET), through studies of three experimental systems: What are the structural and dynamical features that control interfacial recognition and docking between ET partners? What are the structural, dynamical, and energetic features that control the actual electron-transfer event within the protein-protein complex? Mixed-metal hemoglobin (Hb) hybrids provide an unmatched opportunity to study the ET event within a 'pre-docked' complex, across a crystallographically defined, experimentally manipulable protein-protein (inter-subunit) interface. Cytochrome c Peroxidase (CcP) is the paradigm of a protein with multiple, interacting redox centers and multiple binding domains for reacting with its ET partner, cytochrome c (Cc). The physiologically important ET complexes of cytochrome b5 (b5) with myoglobin (Mb) and Hb present complementary issues: The [Mb, b5] system of low molecular weight partners raises fundamental issues of interfacial recognition and docking; the [Hb, b5] pair offers the opportunity to probe the coupling of ET to quaternary assembly and conformation of a multi-subunit protein.