TRANSMISSION AND GENETICS OF ALCOHOL DISORDERS IN A NATIVE AMERICAN TRIBE

使用全基因组扫描，我们将评估乔克托美洲印第安人酗酒和相关精神疾病的遗传联系。乔克托是北美东部的一个大印第安部落，在俄克拉荷马州的部落边界内居住着3万多名登记成员。与酗酒率很高的邻近美洲印第安部落相比，这个部落与众不同，因为酗酒率很低，女性和男性分别约为1%和10%。通过研究低酒精含量的美洲印第安人，我们可以对酒精中毒的遗传和/或环境决定因素的作用有不同的见解。遗传分析将使用来自该部落的三个样本进行：一个小样本随机样本（N=100）， 3个大家庭（每个家庭N bb80）和150多个混合核心家庭的样本。混合的核心家庭将被选择有一些欧美血统和至少一个酗酒的家庭成员。传递/不平衡检验（TDT）是主要利益的连锁分析方法，因为它已被证明随着人口的混合而增加。然而，采样设计也将适应标准的非参数两点和多点联动方法。为了进行上述分析，已经并将继续收集个人精神病学访谈和血液样本。研究诊断是通过精神病学访谈做出的，用于基因分型的DNA正在从血液样本中提取。对于连锁分析，随着技术的发展，我们预计将在整个人类基因组中发现多达2000个独特的序列DNA多态性。在过去的一年里，随机抽样和大家庭的收集工作已经完成。目前正在进行数据库检查和流行病学分析。-基因定位（人类），神经科学，饮酒模式/原因，分子遗传学-人类受试者：访谈，问卷调查或仅限调查

Using a whole genome scan, we will assess genetic linkage to alcoholism and associated psychiatric disorders in Choctaw American Indians. Choctaw is a large Eastern North American Indian tribe with over 30,000 enrolled members living within tribal boundaries in Oklahoma. By contrast to neighboring American Indian tribes that have high prevalences of alcoholism, this tribe stands out because alcoholism has a low prevalence, about 1% and 10% of females and males, respectively. By studying American Indians in the context low alcoholsim, we can expect to reveal different insights into the roles of genetic and/or environmental determinants of alcoholism. Genetic analysis is to be conducted using three samples from the tribe: a small random sample (N=100), 3 large extended families (N>80 per family), and a sample of more than 150 admixed nuclear families. The admixed nuclear familes will be selected to have some Euro-american ancestry and at least one alcoholic family member. The transmission/disequilibrium test (TDT) is the linkage analysis method of principal interest, because it has been shown to have increased power with population admixture. However, the sampling design will also accomodate standard non-parametric two- and multi-point linkage methods. In order to perform the analyses outlined above, individual psychiatric interviews and blood samples have been and continue to be collected. Research diagnoses are made from the psychiatric interviews and DNA for genotyping is being extracted from the blood samples. For the linkage analysis, we expect to type up to 2000 unique sequence DNA polymorphisms spanning the entire human genome as the technology becomes available. In the past year, collection of the random sample and extended families has been completed. Database checks and epidemiological analyses are now under way. - gene mapping (human), neurosciences, drinking patterns/causes, molecular genetics - Human Subjects: Interview, Questionaires, or Surveys Only