MULTIDAY EXPOSURE TO O3 IN ASTHMA

描述：(改编自《调查者摘要》)最新数据来自 申请人的实验室表明，哮喘患者的 对短期吸入臭氧的炎症反应。目前还不清楚， 然而，炎性反应是否有进展 反复短期接触的哮喘者(如在 许多雾霾事件)。臭氧所致人体炎症的研究进展 哮喘有可能增加急性加重的风险 疾病。大多数数据表明臭氧可以诱导呼吸 健康和哮喘受试者的呼吸道炎症是由 支气管镜检查与支气管肺泡灌洗(BAL)分析 流体。而诱导痰的分析是否已经被确认为 吸入性变应原的作用研究还不够充分 经验证可用于空气污染研究。事实上，早些时候关于 在臭氧暴露研究中使用该技术表明，对 痰(对近端呼吸道进行采样)可能会产生冲突的结果 通过分析BAL液(对远端肺进行采样)。为了 痰诱导试验(SI)在肺炎研究中的应用 臭氧诱导的呼吸道炎症，并评估其影响 哮喘受试者反复短期臭氧暴露的三项研究 建议。研究1旨在直接比较分析归纳 哮喘患者短期臭氧暴露后痰中对BAL液的影响 研究对象。研究2旨在比较从痰中获得的结果 反复短期暴露后进行诱导和支气管镜检查以确定 近端和远端的炎症效应是否不同 阿龙。研究3将检验这一假设，即 哮喘患者臭氧诱导的呼吸道炎症反应 单独或同时使用诱导痰重复短期暴露 诱导痰和支气管镜检查，取决于前两种检查的结果 学习。预计拟议的研究将有助于验证 空气污染研究中的诱导痰问题，并解决是否 哮喘患者急诊科和医院使用率增加 暴露在臭氧中是由于与增强的呼吸道有关的急性加重 发炎。

DESCRIPTION: (Adapted from the Investigator's Abstract) Recent data from the applicant's laboratory suggest that persons with asthma have an enhanced inflammatory response to short-term inhalation of ozone. It is not known, however, whether there is progression of the inflammatory response in asthmatic persons with repeated short-term exposures (as would occur during many smog episodes). Progression of ozone-induced inflammation in persons with asthma is likely to increase the risk of acute exacerbation of the disease. Most of the data demonstrating that ozone induces respiratory tract inflammation in healthy and asthmatic subjects has been generated from studies involving bronchoscopy and analysis of bronchoalveolar lavage (BAL) fluid. While analysis if induced sputum has been validated for the investigation of effects of inhaled allergen, it has not been adequately validated for use in air pollution studies. In fact, early reports of the use of the technique in ozone exposure studies suggest that analysis for sputum (which samples the proximal airways) may yield results that conflict with analysis of BAL fluid (which samples the distal lung). In order to validate the use of sputum induction (SI) in the investigation of ozone-induced respiratory tract inflammation and to asses the effects of repeated short-term ozone exposures in asthmatic subjects, three studies are proposed. Study 1 is designed to directly compare analysis of induced sputum to that of BAL fluid after short-term ozone exposure in asthmatic subjects. Study 2 is designed to compare the results obtained from sputum induction and bronchoscopy after repeated short-term exposures to determine whether inflammatory effects are different in proximal airways and distal lung. Study 3 will test the hypothesis that there is a progression of ozone-induced respiratory tract inflammation in asthmatic subjects with repeated short-term exposure using either sputum induction alone or both sputum induction and bronchoscopy, depending on the results of the first two studies. It is anticipated that proposed research will help to validate the issue of sputum induction in air pollution studies and address whether the increased emergency department and hospital utilization of asthmatic persons exposed to ozone is due to acute exacerbation related to enhanced airway inflammation.