CORE--VIROLOGY

病毒学核心的主要目标是提供病毒学支持 项目1至4和免疫学核心。这将是 实现三个具体目标。目标1。提供标准化的病毒 用于体外试验和猕猴攻击研究的储备物。病毒滴度 攻毒储备将包括SHIV，由病毒科博士Yichen Lu提供 研究所，马萨诸塞州剑桥，项目1至3和艾滋病毒- 2SBL 6669，由G博士提供。Biberfeld，斯德哥尔摩，瑞典，项目4. 此外，HIV-1菌株、牛痘构建体和转化 将培养疱疹病毒并根据需要提供给项目， 免疫学核心遗传学上，通过转染获得的单型病毒 将根据需要提供感染性分子克隆，以确定 免疫反应的精细特异性。IHV有一个图书馆， 感染性分子克隆和合适的载体系统， 这个目的。目标2.评价攻毒恒河猴的 传染性将通过血浆p24/27抗原、病毒 共培养，并通过定量血浆病毒RNA测量。为 项目1至3，NASBA方法，检测SIV gag序列将 被利用项目4使用HIV-2SBL 6669作为攻毒病毒， 具体的NASBA正在由我们的合作者开发。可选择地， 将使用QC-PCR测定该病毒的病毒载量。此外 对于这些方法，可能感兴趣的是确定表型或 突破疫苗接种的病毒的基因型（参见，项目1和 3）。病毒学核心将分离这些病毒并表征相关的 表型和基因型。目标3.进行中和试验 抗体和定量VSF。中和抗体测定将 由病毒学中心执行几个在线检测可以检测到 主要分离株和实验室病毒株的特异性抗体。 病毒学核心还将定量提供的上清液中的VSF活性 免疫学核心这些试验将由Mike Merges先生监督， 他在表演和诠释这类作品方面有着丰富的经验， 测定。

The principal goal of the Virology Core is to provide virological support for Projects 1`through 4 and the Immunology Core. This will be accomplished in 3 specific aims. Aim 1. To provide standardized viral stocks for in vitro assays and macaque challenge studies. Titered viral stocks for challenge will include SHIVs, provided by Dr. Yichen Lu, Virus Research Institute., Cambridge, MA., for Projects 1 through 3 and HIV- 2SBL6669, provided by Dr. G. Biberfeld, Stockholm, Sweden, for Project 4. In addition, strains of HIV-1, vaccinia constructs, and transforming herpes viruses will be grown and provided as needed to the projects and Immunology Core. Genetically, monotypic viruses obtained by transfection of infectious molecular clones will be provided as needed to ascertain the fine specificities of immune responses. The IHV has a library of infectious molecular clones and appropriate vector systems available for this purpose. Aim 2. To evaluate challenged rhesus macaques for infectious. Infection will be monitored by plasma p24/27 antigen, virus co-cultivation, and by quantitative plasma viral RNA measurements. For Projects 1 through 3, a NASBA method that detects SIV gag sequences will be used. Project 4 uses HIV-2SBL6669 as the challenge virus and a HIV-2 specific NASBA is under development by our collaborators. Alternatively, QC-PCR will be used to determine viral loads for this virus. In addition to these methods, it may be of interest to determine the phenotype or genotype of viruses that break through vaccination (c.f., Projects 1 and 3). The Virology Core will isolate these viruses and characterize relevant phenotypes and genotypes. Aim 3. TO carry out assays for neutralizing antibodies and to quantify VSFs. Neutralizing antibody assays will be carried out by the Virology Core. Several assays are online that detect antibodies specific for primary isolates and laboratory strains of virus. The Virology Core will also quantify VSF activity in supernatants provided by the Immunology Core. These assays will be overseen by Mr. Mike Merges, who has extensive experience in the performance and interpretation of such assays.