CORE--ANALYTICAL SERVICES

分析服务核心将在设计中提供投入并 对所有项目进行分析，对本项目进行资助。它还将 负责数据的收集、数据库的维护和维护 临床项目分析。这个核心中的流式细胞术将 便于分析所需的细胞动力学和细胞凋亡 在这份提案中。该设施将与个人互动 研究人员，并为实验设计提供意见 负责所有肿瘤解聚和染色程序， 运行示例，并分析和解释数据。流动 设备本身建议开发新的方法来测量 肿瘤中细胞的种群动力学。尤其是 研究人员将确定凋亡细胞死亡的影响 关于细胞动力学参数的估计及其现有的推广 分析程序，以纳入额外的信息 源于对细胞凋亡率的了解。他们建议， 以前测量过的动力学参数(持续时间 S期，TS；肿瘤潜能倍增时间，Tpot；S期 分数，SPF；标签指数，LI)扩展到包括 检测细胞凋亡率、生长因子、生长因子和 量化表示注定失败的细胞的平均寿命TL。

The analytical Services Core will provide input in the design and analysis of all project, on this program project grant. It will also be responsible for the data collection, data base maintenance and analysis of clinical projects. The flow cytometry in this core will facilitate the analysis of cell kinetics and apoptosis that are required in this proposal. The facility will interact with individual investigators and provide input into experimental design, be responsible for all tumor disaggregation and staining procedures, run the samples, and analyze and interpret the data. The flow facility itself proposes to develop novel methods to measure the population dynamics of cells in tumors. In particular the investigators will define the influence that apoptotic cell death has on the estimates of cell kinetic parameters and extend their existing analytical procedures to incorporate the extra information that derives from knowledge of apoptotic rates. They propose that the kinetic parameters which previously have been measured (duration of S-phase, TS; the tumor potential doubling time, Tpot; S-phase fraction, SPF; labeling index, LI) be extend to include measurements of apoptotic frequency; growth factors, GF; and a quantify representing the mean lifetime of doomed cells, TL.