CORE--CELL CULTURE MEDIA PREPARATION FACILITY

细胞培养上清液制备设施(位于 McArdle实验室)在珍妮特·默茨博士的教职员工监督下。 这个220平方英尺的设施配备了层流食品， 水蒸馏装置、多个过滤装置、天平、 一个PH值计，一个37摄氏度的孵化器和几个冰箱。女士。 凯西·约翰逊准备和消毒细胞培养液，根据 每个调查员的需求。去年她准备了3800多升 包括高糖DMEM、Joklik、S-MEM、DMEM 不含酚红、RPMI-1640和F-12。约翰逊女士还准备了特别的 缺乏特定氨基酸的缓冲液和介质，如DMEM。她投保了 通过测试每批培养基的生长情况来测试其无菌情况 污染物，保存3至4周，直到所有测试证明 不是。在过去的一年里，约翰逊女士还为细菌 酵母菌大约有8150个含有各种营养物质的培养皿， 毒品和指示剂。这些介质分别储存在1/2和1/2的垃圾桶中。 放在721房间的冰箱里。整个McArdle的研究人员 实验室根据需要检查这些介质，并注明数量 这样配料的成本就可以计入 个别研究小组。

The Cell Culture Media Preparation Facility (located in room 721 of the McArdle Laboratory) is under the faculty supervision of Dr. Janet Mertz. This 220 square foot facility is equipped with a laminar flow food, a water distillation apparatus, multiple filtration apparatuses, a balance, a pH meter, a 37 degree Celsius incubator, and several refrigerators. Ms. Kathy Johnson prepares and sterilizes cell culture media according to the needs of each investigator. Last year she prepared more than 3,800 liters of media; these media included DMEM with high glucose, Joklik, S-MEM, DMEM without phenol red, RPMI-1640 and F-12. Ms. Johnson also prepares special buffers and media such as DMEM lacking specific amino acids. She insures the sterility of each batch of medium by testing it for growth of contaminants and holding it for 3 to 4 weeks until all tests prove negative. During the last year, Ms. Johnson also prepared for bacterial and yeast work approximately 8,150 plates containing various nutrients, drugs, and indicators. These media are stored in 1/2 and 1 litter bottles in refrigerators located in room 721. Researchers throughout the McArdle Laboratory check out these media as needed, indicating the quantities taken so the costs of the ingredients can be charged back to the individual research groups.