CORE--MOLECULAR DIAGNOSTICS FACILITY

此核心的目的是提供一个集中的设施，以快速 评估已识别基因在儿童癌症中的潜在作用， 利用初级临床样本和已建立的儿科癌细胞 台词。核心实验室将调查基因的表达水平 半定量RT-PCR法或Northern印迹法检测候选基因 使用初级生物提供的探针和序列进行分析 调查人员。此外，还详细评估了是否存在 候选基因的突变将被执行。这些研究将 涉及对重排、缺失和点突变的评估 使用标准的方法。这些活动的集中化 将允许有效地利用现有的临床材料 并将确保准确的结果不会受到来自 在首席调查员的实验室里克隆的序列。此外, 关于初级患者的详细临床信息的可用性 将允许快速评估临床相关性 有趣的发现。这一核心还将有助于评估和 小鼠体内肿瘤生长的特征 产生了转基因和基因敲除/敲除菌株。

The purpose of this Core is to provide a centralized facility to rapidly evaluate the potential role of identified genes in pediatric cancer, using primary clinical samples and established pediatric cancer cell lines. The Core laboratory will investigate the level of expression of candidate genes by either semiquantitative RT-PCR or Northern blot analysis using probes and sequences provided by the primary investigators. In addition, detailed evaluations of the presence of mutations in candidate genes will be performed. These studies will involve evaluations for rearrangements, deletions, and point mutations using standard methodologies. The centralization of these activities will allow for an efficient utilization of available clinical material and will ensure accurate results free from potential contaminations from cloned sequences in the primary investigator's laboratory. In addition, the availability of detailed clinical information on the primary patients will allow for the rapid assessment of the clinical relevance of interesting findings. This Core will also aid in the evaluation and characterization of neoplastic growths identified in the murine transgenic and knockout/knockin strains generated.