CORE--HLA TYPING

本项目的核心分为两个部分。A部分是HLA 本研究中所有糖尿病患者的分型 建议-主要是在芭芭拉戴维斯糖尿病中心在大学 华盛顿大学内分泌诊所， 威斯康星州医学院的儿科内分泌诊所。在 此外，斯坦福大学将对少数患者进行研究 大学，来自露西尔儿科内分泌诊所 索尔特儿童医院在这个核心中，亨利埃利希博士将使用 基于聚合酶链反应（PCR）的方法对HLA DR B1和DQ进行分型 B1基因型的患者在这项研究中进行研究。由于这三 250至300名新糖尿病患者中， 每年的病人，病人的数量将被类型接近， 数量，尽管不太可能对所有患者进行研究， 那些年龄很小的人将无法捐赠足够的资金， 外周血用于所需的研究。 核心预算B部分是为下列人员提供旅费和住房费： 每个参与机构派出两名调查员， 一个中心地点（如西雅图、丹佛或帕洛阿尔托）用于演示， 结果的比较，实验方案的修改，以及 决定每项研究的哪些方面需要重复，扩展， 或者如果必要的话，中断。

The core for this program project is divided into two parts. Part A is HLA typing of all the diabetic patients to be studied in this research proposal--primarily at the Barbara Davis Diabetes Center at the University of Colorado, the Endocrinology Clinic at the University of Washington, and the Pediatric Endocrinology Clinic at the Medical of Wisconsin. In addition, a small number of patients will be studied at Stanford University, coming from the Pediatric Endocrinology Clinic of the Lucille Salter Children's Hospital. In this core, Dr. Henry Erlich will use polymerase chain reaction (PCR) based methods to type the HLA DR B1 and DQ B1 genotype of the patients to be studied in this study. Since these three major and one minor diabetes clinic between 250 to 300 new diabetes patients per year, the numbers of patients to be typed will approach that number, although it is not likely that all patients will be studied since those of very young age will not be able to donate sufficient amounts of peripheral blood for the studies required. Part B of the core is to provide for travel costs and housing costs for two investigators from each of the participating institutions to travel to a central site (e.g. Seattle, Denver, or Palo Alto) for presentation and comparison of results, modifications of experimental protocols, and decisions as to which aspects of each study need to be repeated, extended, or if necessary, discontinued.