PURCHASE OF ANALYTICAL ULTRACENTRIFUGE

有了这个主要工具提案申请，我们请求资金 购买Beckman Optima XL-I分析超速离心机和亲和力 传感器IAsys光学生物传感器设备，将用于升级和 完成我们物理生化设备的武器库 大分子的结构和相互作用的表征 具有生物重要性的复合体。六个主要研究小组在 俄勒冈大学分子生物学研究所描述了 这些项目将极大地促进特定的项目 设备。已提出的实验室和研究项目 具体如下：彼得·冯·希佩尔实验室(组装 以及复制和转录的调控反应)；弗雷德里克 达尔奎斯特实验室(细菌组装能量学 趋化信号复合体)；Tom Stevens实验室(SNARE的作用 控制酵母膜融合特异性的蛋白质)； 卡洛斯·布斯塔曼特实验室(噬菌体和 转录复合体)；Diane Hawley实验室(组装和功能 RNA聚合酶II转录复合体)；以及格雷戈里·弗林 实验室(Hsp70分子伴侣及其在蛋白质折叠中的作用)。在……里面 此外，分子生物学研究所的其他成员将使用 要求的设备，因为他们的研究项目变得服从于 生物物理特征。

With this Major Instruments Proposal Applications we request funds to purchase a Beckman Optima XL-I Analytical Ultracentrifuge and an Affinity Sensors IAsys Optical Biosensor Apparatus, to be used to upgrade and complete our armamentarium of physical biochemical equipment for the characterization of the structure and interactions of macromolecular complexes of biological importance. Six major research groups within the Institute of Molecular Biology at the University of Oregon have described specific projects that will be greatly facilitated by these items of equipment. The laboratories and research projects that have been proposed specifically are listed as follows: Peter von Hippel laboratory (Assembly and Regulatory Reactions in Replication and Transcription); Frederick Dahlquist laboratory (The Energetics of Assembly of the Bacterial Chemotaxis Signaling Complex); Tom Stevens laboratory (The Role of SNARE Proteins in Controlling the Specificity of Membrane Fusion in Yeast); Carlos Bustamante laboratory (Assembly and Structure of Phage and Transcription Complexes); Diane Hawley laboratory (Assembly and Function of the RNA polymerase II Transcription Complexes); and Gregory Flynn laboratory (Hsp70 Chaperones and Their Role in Protein Folding). In addition, other members of the Institute of Molecular Biology will use the requested equipment as their research projects become amenable to biophysical characterization.