BIOMOLECULAR INTERACTION ANALYSIS INSTRUMENTATION

该提案要求进行Biacore 2000生物分子相互作用分析 (BIA)东田纳西州立大学医学院的系统。 该系统在研究大分子方面有几个优点。 相互作用：可以实时研究分子相互作用，从而 缔合和解离速率以及平衡的测量 结合常数。生物岩心检测可以比 荧光法或吸收法，因为它对质量变化敏感 而且不需要试剂的放射性标记。此外，只有一个 相互作用的分子需要提纯，这样才能研究这项技术 用于研究不纯混合物。 这台仪器将大大提高我们研究分子的能力。 大范围生物分子相互作用的控制机制 系统：(1)葡聚糖与特定分子相互作用的分子方面 可以理解受体及其对巨噬细胞激活的影响 通过碳水化合物与免疫细胞的结合分析以及 通过测量与巨噬细胞相关的第二信使系统 激活。(2)肽类神经递质的重要性可以是 通过识别离散大脑中的特定受体而建立的 领域与特征密切相关的相互作用 神经肽及其特定的受体亚型。(3)预适应 可以通过使用Biaccore来了解兔心肌细胞的 腺苷受体亚型的特征.腺苷的检查 受体亚型目前受到肾上腺素受体可获得性的限制， 而咪唑啉受体可以通过更复杂的筛选来理解 通过使用目前不能使用其他方法获得的动力学分析 技术。

This proposal requests a BIAcore 2000 Biomolecular Interaction Analysis (BIA) system for the East Tennessee State University College of Medicine. This system offers several advantages in studying macromolecular interactions: molecular interactions can be studied in real time, allowing measurement of association and dissociation rates as well as equilibrium binding constants. BIAcore detection can be used more extensively than fluorescent or absorption methods since it is sensitive to mass changes and requires no radio-labeling of reagents. Additionally, only one of the interacting molecules needs to be purified, so the technology can be study for studying impure mixtures. This instrument will greatly improve our ability to study molecular mechanisms controlling interactions of biomolecules in a broad range of systems: (1) Molecular aspects of the interaction of glucans with specific receptors and their affect on macrophage activation can be understood through binding analyses between carbohydrates and immune cells as well as by measurement of second messenger systems associated with macrophage activation. (2) The importance of peptide neurotransmitters can be established through identification of specific receptors in discrete brain areas and characterization of the interaction of closely related neuropeptides with their specific receptor subtypes. (3) Preconditioning of rabbit cardiomyocytes can be understood by using the BIAcore to characterize adenosine receptor subtypes; examination of adenosine receptor subtypes is currently limited by the availability adrenoceptors, and imidazoline receptors can be understood through more complex screening and by using kinetic analysis which is currently unavailable using other technologies.