CORE--ANIMAL

动物核心C将为个人提供各种服务 博莱霉素类药物的利用与评价项目 老鼠。动物核心的具体目标包括：1.提供一个 博莱霉素组分给药小鼠的标准化模型 SCOR计划。将对肺部进行组织学和生化检查。 寻找纤维化的证据。核心将实施潜在的治疗 在此肺小鼠模型中评估它们的活性 纤维化症。2.培育和/或维持转基因小鼠品系 在SCOR计划的各个组成部分中使用。 核心将对博莱霉素发挥各种专门的功能- 为个别项目处理的野生型和转基因动物 这个项目。这些程序包括放置渗透泵，核糖核酸 肺组织分离及Northern印迹原位杂交分析 杂交和免疫组织化学。核心的目标是 提供一个易于访问的动物模型，以探索 肺纤维化的发病机制及治疗。

The Animal Core C will perform a variety of services for individual projects related to the utilization and evaluation of bleomycin-treated mice. The Specific Aims of the Animal Core include: 1. Providing a standardized model of bleomycin-treated mice to other components of the SCOR program. The lungs will be assessed histologically and biochemically for evidence of fibrosis. The Core will administer potential therapeutic agents and assess their activity in this murine model of pulmonary fibrosis. 2. Developing and/or maintaining transgenic mice strains utilized in the various components of the SCOR program. The Core will perform a variety of specialized functions on bleomycin- treated wild-type and transgenic animals for individual projects within the program. These procedures include the placement of osmotic pumps, RNA isolation and Northern blot analysis of lung tissue, in situ hybridization, and immunohistochemistry. The goal of the Core is to provide an easily accessible animal model to explore new ideas in the pathogenesis and treatment of pulmonary fibrosis.