NOVAL HUMAN MACROPHAGE RECEPTOR

我们发现人和大鼠的巨噬细胞 表达通过与[125I]-CGP4221结合检测到的新受体， 一种以前被认为对血管紧张素II有选择性的化合物 AT-2受体亚型。然而，血管紧张素II不起作用。 认出这种新的受体。CgP 42112基因的表达 受体在大脑损伤和神经元死亡后发生，在 循环中的人类单核细胞和巨噬细胞。人的文化 含有cgp 42112的巨噬细胞抑制其产生 促炎细胞因子和金属蛋白的分泌。我们是 继续努力克隆新的巨噬细胞受体形式 从人单核细胞和大鼠体内获得的cDNA文库 脾。

We have found that human and rat macrophages express a novel receptor detected by binding of [125I]-CGP 4221, a compound previously considered selective for the Angiotensin II AT-2 receptor subtype. However, Angiotensin II does not recognize the novel receptor. Expression of the CGP 42112 receptor occurs after brain lesions and neuronal death in reat, and in circulating human monocytes and macrophages. Culture of human macrophages with CGP 42112 results in inhibition of production of pro-inflammatory cytokines and secretion of metalloprotein. We are continuing our efforts to clone the novel macrophage receptor form cDNA libraries obtained from human monocytes and from rat spleen.