DIAGNOSTIC ADJUNCTS TO CYTOPATHOLOGICAL DIAGNOSIS

“免疫细胞化学已被证明是一种有价值的 辅助细胞病理学诊断。细胞病理学 科参与了几个临床相关的研究项目， 其中许多使用细针抽吸（FNA）， 免疫细胞化学提供辅助诊断信息 关于各种肿瘤抗原、HLA抗原或 其他标记。这些研究的部分清单包括：（1） 评估在肿瘤细胞中表达CD 22和CD 19的肿瘤细胞百分比。 FNA标本作为使用单克隆抗体的临床试验的一部分 难治性淋巴瘤的治疗;（2）细胞学诊断和 眼淋巴瘤的免疫表型;（3）表征和 一种新的抗恶性肿瘤鼠单克隆抗体的应用 黑色素瘤，MART-1，随后定量MART-1， 来自恶性黑色素瘤患者的FNA，用于治疗 抗MART-1抗体;（4）形态学和免疫细胞化学 骨髓和外周血样本的评估 检测IV期乳腺癌患者的恶性细胞; (5)细胞学和免疫细胞化学分析 描述了用于鉴别恶性肿瘤的抗体， 腺癌间皮瘤;（6）CD 3定量 恶性黑色素瘤FNA标本中的阳性淋巴细胞 免疫治疗前后的患者;（7） 骨髓和单采标本中的细胞角蛋白抗体， 微小残留病和循环肿瘤细胞诊断 乳腺癌患者。7）免疫细胞化学评价 支气管肺泡灌洗标本中的淋巴细胞群 患者局部暴露于内毒素。. "

"Immunocytochemistry has proved to be a valuable diagnostic adjunct to cytopathologic diagnosis. The Cytopathology Section is involved in several clinically-related research projects, many of which utilize fine needle aspiration (FNA) and immunocytochemistry to provide ancillary diagnostic information regarding expression of various tumor antigens, HLA antigens or other markers. A partial listing of such studies includes: (1) Evaluation of percent of tumor cells expressing CD22 and CD19 in FNA specimens as part of a clinical trial using monoclonal antibody therapy for refractory lymphomas;(2) Cytologic diagnosis and immunophenotyping of ocular lymphomas; (3) characterization and application of a novel murine monoclonal antibody for malignant melanoma, MART-1 with subsequent quantitation of MART-1 in FNAs from malignant melanoma patients for treatment with anti-MART-1 antibody; (4) morphologic and immunocytochemical evaluation of bone marrow and peripheral blood samples for the detection of malignant cells in patients with stage IV breast cancer; (5) cytologic and immunocytochemical analysis of recently describeld antibodies for the differentialtion of malignant mesothelioma from adenocarcinoma;(6) Quantitation of CD3 positive lymphocytes in FNA specimens in malignant melanoma patients before and after immunotherapy;(7) utilization of cytokeratin antibodies in bone marrow and apheresis specimens for minimal residual disease and circulating tumor cell diagnoses in breast carcinoma patients.7) immunocytochemical evaluation of lymphoid populations in bronchoalveolar lavage specimens from patients subsequent to local exposure to endotoxin. ."