INFRARED WAVELENGTH MATRIX ASSISTED LASER DESORPTION MASS SPECTROMETRY

使用UV波长激光照射的MALDI-MS已经成为广泛的 采用的技术用于需要MW的生物应用 肽和蛋白质的测定。 红外波长激光器 辐射很少用于MALDI-MS应用。 一 IR MALDI研究量小的原因是缺乏 一个方便的商业红外波长激光器， 持续时间短。 迄今为止，所有研究均使用Er（YAG）激光器进行 具有2.94 μ m的固定波长和100 μ m范围内的脉冲持续时间 - 200 ns。 光学参量放大器的最新商业应用 振荡器（OPO），允许随时调谐激光波长， 范围为0.7 - 4.0 μ m，强脉冲辐照持续时间为 （6 ns使我们有吸引力计划一个彻底的 重新调查IR MALDI。 IR MALDI的这项调查正在 使用Surelite II Nd（YAG）泵浦激光器与 a Mirage 3000 OPO（Continuum，Santa Clara，CA）。 你... 我们有 成功地将OPO的输出耦合到我们的第二个定制的 MALDI-TOF仪器，并进行了广泛的计划， 用新仪器进行研究。 我们已经做了一些 有趣的发现，报告在一份文件（S。 牛，W。张先生， 和B.T. Chait“红外与紫外的直接比较 波长基质辅助激光解吸质谱法 Proteins”J. Am.质谱学会 9，（1998）1-7）。 这些发现 具有潜在的实用价值和基础价值 （用于获得对MALD-MS过程的理解）。

MALDI-MS using UV wavelength laser irradiation has become a widely adopted technique for biological applications that require MW determination of peptides and proteins. IR wavelength laser irradiation has been much less used for MALDI-MS applications. One reason for the small amount of research in IR MALDI has been the lack of a convenient commercial IR-wavelength laser that produces pulses of short duration. All studies to date carried out using Er(YAG) lasers with a fixed wavelength of 2.94 (m and pulse duration in the range 100 - 200 ns. The recent commercial availability of optical parametric oscillators (OPO) that allow ready tuning of the laser wavelength in the range 0.7 - 4.0 (m and intense pulses of irradiation with duration ( 6 ns has made it attractive for us to plan a thorough re-investigation of IR MALDI. This investigation of IR MALDI is being undertaken using a Surelite II Nd(YAG) pump laser in combination with a Mirage 3000 OPO (Continuum, Santa Clara, CA .). cont.. We have successfully coupled the output of the OPO to our second custom built MALDI-TOF instrument and have carried out an extensive program of research with the new instrument. We have made a number of interesting findings that are reported in a paper (S. Niu, W. Zhang, and B.T. Chait "Direct Comparison of Infrared aand Ultraviolet Wavelength Matrix-Assisted Laser Desorption Mass Spectrometry of Proteins" J. Am. Soc. Mass Spectrom. 9, (1998) 1-7). These findings are of potential value both from a practical and a fundamental point of view (for obtaining an understanding of the MALD-MS process).