AMINO TERMINAL PROCESSING OF YEAST PROTEINS

表达突变型谷胱甘肽-S-转移酶（GST）， 纯化自野生型酵母、MAP 1酵母和MAP 2酵母。 以来 甲硫氨酸氨基肽酶（MetAP）参与N-末端 处理，我们预测，GST表达的酵母菌株缺乏 MetAP 1或MetAP 2不应具有其N-末端蛋氨酸 完全移除。 我们希望用质谱分析 不同GST的MET去除程度，以了解两种GST的作用 不同的元AP。 我们使用了一系列的突变体 谷胱甘肽-S-转移酶，以评估每种蛋氨酸的作用 真核细胞中的氨基肽酶。

Mutant Glutathione-S-Transferases (GST) were expressed and purified from wild-type yeast, map1 yeast, and map2 yeast. Since methionine aminopeptidases (MetAPs) are involved in N-terminal processing, we predict that GSTs expressed in yeast strains lacking either MetAP1 or MetAP2 should not have their N-terminal methionine completely removed. We hope using mass spectrometry to analyze the extent of met-removal of different GSTs to understand the roles of two distinct MetAPs. We are using a series of mutant glutathionine-S-transferases to evaluate the roles of each methionine aminopeptidases in eukaryotic cells.