Functional epigenomics interrogation of DNA methylation dynamics during vertebrate development and evolution

DNA甲基化（mC）是维持正确基因表达模式所必需的表观遗传信号。为了研究脊椎动物胚胎发育和进化过程中mC与转录之间的因果关系，本项目将在硬骨鱼、两栖动物和哺乳动物发育的不同阶段进行高分辨率mC分析。高度保守和同步的甲基化序列将被用作蛋白质组学筛选的诱饵，以鉴定新的5mC“读取器”。mC“读取器”基因组图谱的生成及其与发育mC图谱的整合将揭示脊椎动物胚胎发生过程中的瞬时表观基因组动力学，并为这些关键发育机制的保护提供新的见解。

DNA methylation (mC) is an epigenetic signal essential for the maintenance of correct gene expression patterns. To investigate the causal relationships between mC and transcription during vertebrate embryonic development and evolution, this project will perform high-resolution mC profiling at different stages of teleost, amphibian and mammalian development. Highly conserved and syntenic, methylated sequences will then be used as baits in proteomics screens to identify novel 5mC 'readers'. The generation of genomic profiles of mC 'readers' and their integration with developmental mC maps will reveal transient epigenome dynamics during vertebrate embryogenesis and provide new insights into the conservation of these crucial developmental mechanisms.