CHARACTERIZATION OF CARBORANE CONTAINING POLYPEPTIDES

我们有合成和邻位碳硼烷氨基酸，并计划 描述其插入的结构效应 同源多氨基酸，如聚丙氨酸或聚苯丙氨酸。这个 这些多肽的二级结构应该是螺旋的，具有 由空间效应引起的碳硼烷微扰。与之比较 其他β-氨基酸插入，如邻氨基苯甲酸 这些多肽将使我们能够表征特定的螺旋断裂 碳硼烷的相互作用。螺旋结构的变化引起的 通过插入这些类型的氨基酸，最初将 用圆二色谱测定，再用二维核磁共振测定。 使用MidasPlus进行建模将使我们能够预测 修饰的多肽，将有助于我们选择有用的模型 待进一步研究的化合物。

We have synthesize and ortho-carborane amino acid and plan to characterize the structural effects of its insertion into homopolyaminoacids such as poly-Alanine or poly-Phenylalanine. The secondary structure of these peptides should be helical, with perturbation by the carborane due to steric effects. Compaison with other beta-amino acid insertions such as ortho-aminobenzoic acid in these peptides will allow us to characterize specific helix-breaking interactions of the carborane. The change in helical structure caused by insertions of these types of amino acids will be initially determined using circular dichroism spectroscopy then by 2-D NMR. Modeling using MidasPlus will allow us to predict the structure of the modified polypeptides, and will aid us in selecting useful model compounds for further studies.