BIOLOGICAL METAL CLUSTERS BIOPHYSICAL & MODEL STUDIES

醇、醛和酮的相互转化是必不可少的 在原核生物和真核生物中的过程。 的氧化还原酶 催化这些反应使用各种不同的电子 受体，可以分为三个主要群体：1。 的 NAD（P）依赖性乙醇脱氢酶2。 NAD（P）-独立 醇脱氢酶，其使用其它辅因子进行催化;以及 3. FAD依赖性醇氧化酶，催化不可逆 醇的氧化。 H Thermoanaerobic（原 布氏嗜热厌氧菌乙醇脱氢酶（TBADH）是一种 链，NADP连接的，A类酶H，可逆地催化 将仲醇氧化成相应的酮。 H The 普遍存在的乙醇脱氢酶（ADH）不仅在Hin 细菌，但也在酵母，植物，昆虫和人。ADH是一种 氧化还原酶，需要NAD（H）或NADP（H）作为辅酶， 与初级和次级、线性和支链反应， 脂族和芳族醇及其相应的醛 和酮。 虽然一些ADH依赖于铁的活化， 已知某些ADH是不含金属的，大多数ADH含有锌， Hactive site. 锌依赖性ADH要么是二聚体，通常存在于 高等植物和哺乳动物，或四聚体，如存在于 酵母菌和细菌。 从酵母菌中分离到一种单体ADH Hcerevisiae，但其金属含量尚不清楚。

Interconversion of alcohols, aldehydes, and ketones are essential processes in both prokaryotes and eukaryotes. The oxidoreductases catalyzing these reactions use a variety of different electron acceptors and can be divided into three main groups: 1. the NAD(P)-dependent alcohol dehydrogenases 2. the NAD(P)-independent alcohol dehydrogenases, which use other cofactors for catalysis; and 3. FAD-dependent alcohol oxidases, which catalyze irreversible oxidation of alcohols. H Thermoanaerobacter (formerly Thermoanaerobium) brockii alcohol Hdehydrogenase (TBADH) is a medium chain, NADP-linked, class A enzyme Hthat reversibly catalyses the oxidation of secondary alcohols to the Hcorresponding ketones. H The ubiquitous alcohol dehydrogenases (ADHs) are found not only Hin bacteria, but also in yeast, plants, insects and in man. ADH is an oxidoreductase, requiring either NAD(H) or NADP(H) as a coenzyme, that reacts with primary and secondary, linear and branched-chain, aliphatic and aromatic alcohols and with their corresponding aldehydes and ketones. Although some ADHs depend on iron for activation and certain ADHs are known to be metal-free, most ADHs contain zinc at the Hactive site. Zinc-dependent ADHs are either dimers, usually found in Hhigher plants and mammals, or tetramers, such as those present in Hyeast and bacteria. A monomeric ADH was isolated from Saccharomyces Hcerevisiae, but its metal content is yet unknown.