STRUCTURAL PARAMETERS INVOLVED IN METAL RECOGNITION

金属识别涉及的结构参数

• 批准号: 8362356
• 负责人: MICHAEL J MARONEY
• 金额: $ 0.19万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362356
• 关键词: Antibiotics; Biological; Cells; Escherichia coli; Funding; Grant; Helicobacter pylori; Metal Ion Binding; Metals; National Center for Research Resources; Nature; Nickel; Pattern; Principal Investigator; Proteins; Radiation; Research; Research Infrastructure; Resources; Source; Structure; System; United States National Institutes of Health; cost; design; research study; response; structural biology; tool; trafficking

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The proposed XAS experiments are part of a project designed to examine how nature designs proteins to recognize one metal (or small group of metals) from all the metals present in cells, and generate specific biological responses. Proteins involved in nickel trafficking in E. coli and H. pylori were chosen because of the desire to examine an entire trafficking system (simplicity of the nickel traffic pattern) in order to understand the general features of specific metal recognition, and because of the potential for antibiotic strategies involving interference with bacterial nickel trafficking. XAS provides the main structural tool for examining the structure of cognate and non-cognate metal ions bound to metallotransporters, metallochaperones, and metalloregulators.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 拟议的XAS实验是一个项目的一部分，该项目旨在研究大自然如何设计蛋白质，以识别细胞中存在的所有金属中的一种金属（或一小群金属），并产生特定的生物反应。镍转运蛋白在大肠杆菌中的表达。coli和H.选择幽门螺杆菌是因为希望检查整个运输系统（镍运输模式的简单性），以便了解特定金属识别的一般特征，并且因为涉及干扰细菌镍运输的抗生素策略的潜力。XAS为检查与金属转运蛋白、金属伴侣和金属调节剂结合的同源和非同源金属离子的结构提供了主要的结构工具。