DIFFRACTION ANALYSIS OF FLP RECOMBINASE & ANIMAL LECTINS

在过去一年，我们完成了修订和出版 C3 d是CR2受体的C3片段和配体（见下文的《科学》）。 通过在来自数据的Se边缘处的MAD定相来解析结构 收集在国际象棋。 该结构含有8个硒原子， 与大卫史密斯博士合作，数据被用来 说明SnB法测定硒的实用性 下部结构 该结构为C3的活化提供了模型 以及深入了解与CR2相互作用的基础 受体的 我们亦已完成修订及出版 人半乳糖凝集素-3与乳糖复合物CRD， N-乙酰乳糖胺来自于在CHESS收集的数据。 这些分子 在调节细胞-细胞和细胞-基质相互作用中重要。 我们 结构定义了碳水化合物结合的基础， 另外提供了对寡聚化模式的洞察，如 这个凝集素。 此外，我们还在CHESS上收集了C型 凝集素和CRE重组酶-DNA复合物的晶体。 在这两种情况下 这些结构的解决正在进行中。

In the past year we completed the refinement and publication of C3d a C3 fragment and ligand for the CR2 receptor (see Science below). The structure was solved by MAD phasing at the Se edge from data collected at CHESS. The structure contains 8 selenium atoms and in collaboration with with Dr. David Smith, the data was used to illustrate the utility of the SnB method for determining the selenium substructure. The structure provides a model for the activation of C3 as well as insight into the basis for interaction with the CR2 receptor. We have also completed the refinement and publication of the CRD of human galectin-3 in complex with lactose and N-Acetylactosamine from data collected at CHESS. These molecules are important in modulating cell-cell and cell-matrix interactions. Our structure has defined the basis for carbohydrate-binding, and in addition provided insight into the mode of oligomerization, shown by this lectin. In addition, we collected data at CHESS on a C-type lectin and crystals of a CRE recombinase-DNA complex. In both cases the solution of these structures is in progress.