BIOPHYSICAL STUDIES OF CYTOSKELETAL FUNCTION

描述：拟议的实验的目标是解决两个广泛的问题 问题。非肌肉组织细胞如何调节收缩力 它们相互作用，并作用于它们所在的细胞外基质 嵌入？这些细胞能在多大程度上以及如何感知外力 施加在它们身上，并以生化和机械的方式做出反应。六个目标是 建议：(1)确定不同信号通路的贡献 肌球蛋白的激活和收缩力量的发挥；(2) 确定微管破裂如何导致肌球蛋白激活 网络；(3)确定磷脂酶C Delta1的途径 有助于产生成纤维细胞的收缩力；(4) 确定选定的肌动蛋白细丝封端蛋白在 控制肌动蛋白细胞骨架的硬度；(5)确定如何 与细胞外基质的黏附有助于组织的发育和发挥 力；以及(6)确定对细胞施加的力是否通过 整合素-配体相互作用具有重要的调节功能。

DESCRIPTION: The goal of the proposed experiments is to address two broad questions. How do non-muscle tissue cells regulate the contractile forces they exert on each other and on the extracellular matrices in which they are embedded? To what extent and how can these cells sense external forces exerted on them and respond biochemically and mechanically. Six goals are proposed : (1) to determine the contribution of different signaling pathways to the activation of myosin and the exertion of contractile force; (2) to determine how myosin activation results from disruption of the microtubule network; (3) to determine the pathway by which phospholipase C delta1 contributes to generation of contractile force in fibroblasts; (4) to determine the roles of selected actin filament capping proteins in controlling the stiffness of the actin cytoskeleton; (5) to determine how adhesion to the ECM contributes to development of tissues and the exertion of force; and (6) to determine whether the exertion of force on cells via integrin-ligand interactions has important regulatory functions.