COMPARATIVE ANALYSIS OF GROUP A AND B STREPTOCCAL GENOME

这个FIRCA提案是两个参与者之间的合作 在病原学研究方面有共同兴趣的机构 链球菌。实验医学研究所（IEM）在圣。 俄罗斯彼得堡是世界卫生组织的国家中心， 链球菌和微生物学和免疫学系 俄克拉荷马州大学健康科学中心（OUHSC）因 链球菌遗传学研究。目前的 A组链球菌（GAS，化脓性链球菌）全序列 俄克拉荷马州大学正在确定基因组。这项工作是 由国家卫生研究院资助给费莱蒂博士。IEM最近 启动了一个项目，建立该群体的物理和遗传图谱 B链球菌基因组（GBS，无乳链球菌）。这个有机体 像GAS一样，具有相当大的医学重要性， 其完整序列将对理解那些 与其毒力相关的因素，以及设计新的方法， 通过药物治疗的新靶点或开发新的 疫苗。因此，GBS的完整序列的确定 基因组将具有极大的兴趣和价值。然而，这样的项目 在FIRCA应用方面成本过高;因此， 研究者在本申请中提出了一种替代方法。一个伟大 关于GAS和GBS的遗传相关性存在大量数据 尽管他们在临床症状和疾病方面存在差异。具体 这项建议的目的是确定那些基因序列， 这两种生物体之间的差异和独特性， 他们这种方法是可能的，使用生物技术， 可以与独特的GBS DNA分离 序列的然后可以鉴定、克隆和鉴定独特的GBS序列。 这项工作将由OUHSC提供。这个项目的意义 这一方法可以作为其他国家经济有效模式， 对近缘物种的基因组分析感兴趣。此外，NE 信息将立即提供给科学界， 开始重要的生物学研究工作， 负责疾病的GBS基因组和疾病设计的步骤 预防最后，这两个机构及其调查人员有一个 通过互动和联合方式开展国际合作的良好记录 公布结果。

This FIRCA proposal is a collaboration between two participating institutions with a common interest in the study of pathogenic streptococci. The Institute of Experimental Medicine (IEM) in St. Petersburg, Russia, is a World Health Organization national center for streptococci and the Department of Microbiology and Immunology of the University of Oklahoma Health Sciences Center (OUHSC) is well known for its studies on the genetics of streptococci. At the present time, the complete sequence of group A streptococcus (GAS, Streptococcus pyogenes) genome is being determined at the University of Oklahoma. This work is sponsored by a NIH grant from NIAID to Dr. Ferreti. The IEM has recently initiated a project to establish a physical and genetic map of the group B streptococcal genome (GBS, Streptococcus agalactiae). This organism, like that of GAS, is of considerable medical importance and knowledge of its complete sequence would be of great interest in understanding those factors associated with its virulence as well as in designing new ways for its control via either new targets for drug therapy or development of new vaccines. Thus, the determination of the complete sequence of the GBS genome would be of great interest and value. However, such a project is cost prohibitive in terms of a FIRCA application; therefore, the investigators propose an alternative approach in this application. A great deal of data exists concerning the genetic relatedness of the GAS and GBS despite their differences in clinical symptoms and disease. The specific aims of this proposal are to identify those gene sequences that are different and unique between the two organisms, and to clone and sequence them. Such an approach is possible using the technique of a organisms hybridize with one another and can be separated from unique GBS DNA sequences. The unique GBS sequences can then be identified, cloned, and this work will be provided by the OUHSC. The significance of this project is that this approach could serve as a cost effective model for other interested in genome analysis of closely related species. Additionally, ne information will be immediately available to the scientific community to begin the important work of biological investigation into those factors of the GBS genome responsible for disease and steps designed for disease prevention. Finally, the two institutions and their investigators have a proven record of international cooperation by way of interaction and joint publication of results.