TRANSFER RNA RECOGNITION BY CLASS II SYNTHETASES

描述 这是一个更新的建议，比较研究的细胞质和 来自酵母和植物的细胞器丝氨酰tRNA合成酶，拟南芥 和玉米。 具体目标包括克隆、测序和表达 线粒体丝氨酰tRNA合成酶。酿酒酵母，叶绿体 来自玉米的SerRS和来自拟南芥的细胞质SerRS。 基板 各种同工酶的结合结构域将通过定点酶联免疫吸附试验进行分析。 诱变 另一个目标是鉴定 丝氨酰-tRNA负责被其同源物识别 合成酶

DESCRIPTION This is a renewal proposal for a comparative study of cytoplasmic and organelle seryl tRNA synthetases (SerRS) from yeast and plants, Arabidopsis and maize. Specific aims include the cloning, sequencing and expression of the mitochondrial seryl tRNA synthetase from S. cerevisiae, the chloroplast SerRS from maize and the cytoplasmic SerRS from Arabidopsis. Substrate binding domains of the various isozymes will be analyzed by site-directed mutagenesis. An additional goal is the identification of the nucleotides in seryl-tRNA's which are responsible for recognition by their cognate synthetases.