Core--Immunology

Immunology Core将进行多参数流式细胞术检测和细胞因子检测。卡蒙博士，将是这个中心的主任。他是宾夕法尼亚大学医学中心临床免疫学实验室主任。临床免疫学实验室为自身免疫性疾病患者提供广泛的实验室检测，包括多发性硬化症和SLE，以及先进的多参数四色流式细胞术。在接受抗体治疗的MS和SLE患者中，CD4+和CD8+ T细胞和B细胞亚群的改变可以被确定。通过观察CD4+、CD8+或B细胞中一系列活化标记物和粘附分子的表达，可以评估淋巴细胞的活化情况。我们实验室最近建立了全血单细胞细胞因子测定法，通过通透化细胞的细胞内染色来分析组成型和诱导型细胞因子的产生。这种方法可以识别产生细胞因子的细胞，同时分析表面表型。细胞因子的体外生产也可以用elisa法测定。我们可以测量IL-2、IL-4、IL-10、IL-12 （p40和具有生物活性的p70异源二聚体）、inf - γ、tnf - α和lt - α。实验室使用的所有试剂在使用前都要经过质量控制程序。所有质量控制程序的结果由主管审核，必要时实施纠正措施。我们的实验室参与QA项目，包括CAP季度调查和CDC T细胞免疫表型性能评估项目。流式细胞仪的性能监测每天使用悬浮珠来演示散射，荧光，并在规定的范围内补偿参数。本部门聘用的技术人员均符合CLIA88指导方针的要求，具备执行“高复杂性测试”的资格。实验室主任和主管分别作为主任和技术主管满足CLIA 88的要求。实验室为所有员工保持全面的安全计划，包括OSHA法规，并符合所有联邦，州和地方法规。

The Immunology Core will perform multi-parameter flow cytometry assays and cytokine assays. Dr. Kamoun, will be the Director of this core. He is the Director of the Clinical Immunology Laboratory at the University of Pennsylvania Medical Center. The Clinical Immunology Laboratory provides a wide range of laboratory tests on patients with autoimmune diseases including MS, and SLE as well as advanced multi- parameter four-color flow cytometry-based assays. Alterations in subset of CD4+ and CD8+ T cells and B cells can be determined in MS and SLE patients treated with antibody therapies. Lymphocyte activation can be evaluated assessing expression CD4+, CD8+ or B cells of a series of activation markers and adhesion molecules. Analysis of constitutive and induced cytokine production by intracellular staining of permeabilized cells can be performed using whole blood single cell cytokine assays which was recently established in our laboratory. This method allows the identification of cells producing cytokine while simultaneously analyzing the surface phenotype. In vitro production of cytokine can also be measured using ELISA-based assays. We can measured IL-2, IL-4, IL-10, IL-12 (p40 and the biologically active p70 heterodimer), INF-gamma, TNF-alpha and LT-alpha. All reagents used in the Laboratory are subjected to quality control procedures prior to use. All results of QC procedures are reviewed by the supervisor with corrective action implemented as necessary. Our lab participates in QA programs including the CAP surveys quarterly and in the CDC T cell immunophenotyping performance evaluation program. Performance of the flow cytometers is monitored daily using a suspension of beads to demonstrate scatter, fluorescence, and compensation parameters within defined limits. The Technologists employed in this section of the laboratory are each qualified under CLIA88 guidelines to perform "high complexity testing". The Laboratory Director and the Supervisor meet CLIA 88 requirements as director and technical supervisor respectively. The laboratory maintains a comprehensive safety program for all employees encompassing OSHA regulation and conforming to all Federal, State and local regulation.