FUNDAMENTALS OF BIOLOGICAL ELECTRON TRANSFER

描述：氧化还原酶功能障碍被认为是 对几种常见疾病的衰弱做出了重大贡献 是高度复杂的，治愈的希望很小。了解 不同类型氧化还原酶的作用原理，以及 他们的工程公差和失败的门槛几乎 当然有助于改善疾病的影响，通过 提供急需的诊断工具，并将方向指向 解药。这项建议旨在了解 许多氧化还原酶，即电子的来往传递 催化或能量耦合位置。国际和平研究所计划应用共识 以及从我们对天然氧化还原蛋白的检测中提取的一般性 结构和电子隧道功能对设计的影响，从头开始 简化氧化还原蛋白的合成和组装，即模板。 通过检查相对简单的稳定性中的自然氧化还原中心， 具有结构特征的、多相的多肽环境 Maquettes，他希望描述与之相关的基本事件 常见氧化还原辅因子的氧化还原平衡。 氧化还原协因数将分别和结合在一起进行研究 在多个氧化还原辅因子链模型中。PI计划激活 通过这些链的蛋白质内电子和自由基转移 在电极/多肽上用光、氧或电方法 单层界面。这些模型被设计成最终访问 控制电子转移的动力学因素，并揭示了 平衡性质在动力学时间标度上表现出来。

DESCRIPTION: Oxidoreductase dysfunction is becoming recognized as a major contribution to the debilitation of several common diseases that are highly complex with poor prospects for cure. Understanding the principles of function of the different classes of oxidoreductases, and their engineering tolerances and the thresholds of failure would almost certainly help in ameliorating the effects of the illnesses, by providing much needed diagnostic tools and pointing the direction to a cure. This proposal is aimed at understanding a fundamental part of many oxidoreductases, namely the delivery of electrons to and from catalytic or energy coupling sites. The PI plans to apply the consensus and generality extracted from our examination of natural redox protein construction and electron tunneling function to the design, de novo synthesis and assembly of simplified redox proteins; i.e., maquettes. By examing natural redox centers in the relatively simple stable, structurally characterized, heterogeneous peptide environment of maquettes, he hopes to describe the basic events that are coupled at equilibrium to the oxidation and reduction of common redox cofactors. Redox cofactors will be examined, both individually, and when integrated in multiple redox cofactor chain maquettes. The PI plans to activate intraprotein electron and radical transfer through these chains with light, oxygen or with electrical methods at the electrode/peptide monolayer interface. These maquettes are designed to ultimately access the kinetic factors that govern electron transfer and reveal how the equilibrium properties manifest themselves on the kinetic timescale.