LIPOSOME MEDIATED GENE TRANSFER

描述：(改编自申请者摘要)这一阶段STTR的目标 应用是评价三方转染复合体(A)的使用 表达靶基因的质粒、阳离子脂质体和复制 用于内溶的缺陷腺病毒)将转基因运送到支气管 体外和体内的上皮细胞。我们的目标是尽量减少病毒感染 通过化学交联腺病毒到阳离子的毒性 和/或通过在三方中使用更弱的腺病毒 很复杂。这些新的三方复合体将从水平和持续时间两方面进行评估 在培养的人支气管细胞和在小鼠体内的表达 模型系统。 建议的商业应用：不可用

DESCRIPTION: (adapted from applicant's abstract) The goal of this phase I STTR application is to evaluate the use of tripartite transfection complex (a plasmid expressing a target gene, cationic liposomes, and a replication defective adenovirus for endosomolysis) to deliver transgenes to bronchial epithelial cells both in vitro and in vivo. The goals are to minimize viral toxicity by either chemically crosslinking the adenoviruses to the cationic liposomes and/or by using a more attenuated adenovirus in the tripartite complex. These new tripartite complexes will be evaluted for level and duration of expression both in human bronchial cells in culture and in vivo in a murine model system. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE