SEARCHbreast: A virtual resource to facilitate sharing breast cancer materials and knowledge to benefit the 3Rs

我们将开发一个可搜索的与乳腺癌相关的动物资源数据库，科学家可以共享，以潜在地减少重复，同时提供对未充分利用的档案材料资源的访问。虽然是虚拟资源，但我们期待网络伙伴和新研究人员在共享材料方面进行有价值的交叉合作，而不是复制模型来产生这种合作。鉴于此类在线资源提供的信息的敏感性，我们认识到可能存在担忧，因此这将通过安全密码保护来实现。这将由在线技术专业知识补充，并附带详细的标准操作规程，例如，如何估计基因工程小鼠（GEM）和乳腺癌移植模型所需的动物数量，以及如何最好地收集和保存材料，从而减少浪费，改进正在进行的工作，并对未来的研究产生积极影响。NC3Rs已经改进了动物实验报告（ARRIVE指南）。我们的目标是更进一步，开发一个基于网络的生物信息学搜索门户，允许研究人员挖掘与乳腺癌相关的已发表的体内研究，以尽量减少以前工作的重复，并访问公共存储库中的数据。这与DFCI-MGI、KOMP等其他资源不同，后者侧重于收集/存储广泛疾病类型的单基因/等位基因敲除，而不是用于单一疾病研究的模型类型的综合目录。我们将举办一系列研讨会，将研究人员聚集在一起，分享想法和资源。这些活动将轮流由每个中心主办，允许全英国的科学家参加。第一次将在项目的前6个月内进行，向英国乳腺癌研究社区介绍我们的搜索乳房愿景。工作坊形式将包括4-6次演讲，然后是分组讨论。随后的研讨会将以主题为主题，包括如何选择最佳的体内模型来解决特定的研究问题，使用生物信息学门户网站，如何建立3D细胞培养模型作为动物替代品等信息。我们将邀请一位国际嘉宾参加其中至少一次研讨会，以扩大SEARCHbreast的影响。我们将举办一个为期一天的nc3rs与英国癌症研究协会（BACR）联合研讨会，向更广泛的癌症研究界推广SEARCHbreast蓝图。Holen博士是BACR会议和培训委员会的成员，该委员会负责指导和鼓励这类倡议。此外，我们将提供与乳腺癌运动组织库的联合研讨会，提供关于如何开发和使用从人类乳腺组织开发的替代3D多细胞组织培养模型来研究乳腺生物学的建议/培训。一系列的网络研讨会将在英国以外推广SEARCHbreast。研讨会和网络研讨会将广泛宣传。一旦与NC3Rs合作在乳腺癌中建立，我们将安排与其他资助机构（如MRC， CRUK，英国前列腺癌，LLR，英国心脏研究，英国帕金森等）的会议，以展示我们的资源可能对其他疾病类型的3Rs产生的影响。这些会议将由一名申请人和协调员参加。虽然大多数资助机构现在将数据共享作为资助的常规条件，但我们将与这些机构/期刊合作，旨在将我们改进的数据共享概念嵌入到他们的申请/提交过程中。我们的蓝图具有足够的通用性，因此可以很容易地用于其他疾病，其首字母缩略词经过调整以反映这一点，同时保持与原始的一致性，例如SEARCHcardiovascular， SEARCHlung等。鉴于nc3r在哮喘研究上的大量投资，SEARCHlung可能对它们特别有吸引力。我们将积极与这组研究人员合作，以促进这一点。

We will develop a searchable database of animal resources related to breast cancer that scientists can share to potentially minimise repetition, at the same time providing access to an under-used resource of archival material. Whilst a virtual resource we anticipate valuable cross collaborations between network partners and new researchers in sharing materials rather than duplicating models to generate such. We recognise there may be concerns given the sensitive nature of the information provided by such an on-line resource hence this would be achieved via secure password protection. This will be complemented by on-line technical expertise accompanied by detailed SOPs describing e.g. how to estimate the number of animals needed in genetically engineered mouse (GEM) and transplantation models of breast cancer and how best to collect and preserve material thereby reducing waste, refining ongoing work and impacting positively on future studies. NC3Rs has already improved the reporting of animal experiments (ARRIVE guidelines). We aim to take this a step further by developing a web based bioinformatics search portal to allow researchers to mine published in vivo studies related to breast cancer to minimise repetition of previous work and access data placed in public repositories. This differs from other resources e.g. DFCI-MGI, KOMP which focus on collection/ storage of single gene/allele knockouts across a broad range of disease types rather than a comprehensive catalogue of the types of model available for study in a single disease. We will hold a series of workshops bringing researchers together to share ideas and resources. These will be hosted by each centre in turn, allowing attendance by scientists throughout the UK. The first will take place within the first 6 months of the project to introduce the UK breast cancer research community to our SEARCHbreast vision. Workshop format will include 4-6 presentations, followed by breakout discussion groups. Subsequent workshops will be themed and could include how to select the best in vivo model to address a specific research question, use of the bioinformatics portal, information on how to establish 3D cell culture models as animal alternatives etc. We will invite one international guest speaker to at least one of these workshops, broadening the SEARCHbreast impact. We will organise a joint NC3Rs-British Association of Cancer Research (BACR) one day workshop to promote the SEARCHbreast blueprint to the wider cancer research community. Dr Holen sits on the BACR Meetings and Training Committee which directs and encourages this sort of initiative. Additionally, we will offer a joint workshop with the Breast Cancer Campaign Tissue Bank, providing advice/training on how to develop and use alternative 3D multicellular tissue culture models developed from human breast tissue to study breast biology. A series of webinars will promote SEARCHbreast beyond the UK. Workshops and webinars will be advertised widely. Once established in breast cancer, in collaboration with NC3Rs, we will arrange meetings with other funding bodies e.g. MRC, CRUK, Prostate Cancer UK, LLR, Heart Research UK, Parkinsons UK etc, to demonstrate the benefits our resource could have impacting on the 3Rs across other disease types. These will be attended by one of the applicants and the coordinator. While most funding agencies now implement data sharing as a routine condition of funding, we will work with these agencies/journals aiming to embed our improved data sharing concept into their application/submission process. Our blueprint is sufficiently generic such that it could be adopted easily for other diseases with the acronym adapted to reflect this while retaining uniformity with the original e.g. SEARCHcardiovascular, SEARCHlung etc. SEARCHlung may be particularly attractive to the NC3Rs given their substantial investment in asthma research. We will actively engage with this group of researchers to promote this.