NOVEL P36 MBP KINASE IN CERAMIDE-INDUCED APOPTOSIS

描述(改编自调查人员摘要)：私家侦探之前 证明了一个36 kDa的髓鞘碱性蛋白激酶(MBP Kinase)是 神经酰胺诱导HL60早幼粒细胞白血病细胞凋亡过程中的激活 细胞。该激酶在一组细胞中被激活，这些细胞实际上 启动了细胞凋亡。纯化和显微测序显示， 一种来自先前发现的哺乳动物不育蛋白的蛋白激酶 (MST)Kinase，其两种亚型被克隆，并通过 P.I.P.I.现在计划：1)描述细胞调节的特征 MST激酶对应激/凋亡信号的响应；2)表征 利用可诱导表达系统研究这些激酶的生理作用 和基因工程突变体，以及3)表征信号 导致MST激酶激活的途径。

DESCRIPTION (adapted from investigator's abstract): The P.I. has previously demonstrated that a 36kDa Myelin Basic Protein Kinase (MBP Kinase) is activated during ceramide-induced apoptosis in HL60 promyelocytic leukemia cells. This kinase is activated in the subset of cells that have actually initiated apoptosis. Purification and microsequencing reveal that the kinase is derived from a previously identified Mammalian Sterile Twenty (MST) Kinase, two isoforms of which were cloned and characterized by the P.I.. The P.I. now plans to: 1) characterize the cellular regulation of MST kinases in response to stress/apoptotic signals; 2) characterize the physiological roles of these kinases using an inducible expression system and genetically engineered mutants and 3) characterize the signalling pathway that leads to activation of the MST kinases.