CARCINOGENIC/TOXICOLOGIC EFECTS OF PHIP AND DIETARY FAT ON RAT MAMMARY GLAND

为了更好地了解乳腺癌的发病机制， 食源性杂环胺2-氨基-1-甲基-6- 苯基咪唑[4，5-B]吡啶（PhIP）和膳食脂肪的影响， 正在进行研究以评估PhIP诱导的早期改变， 大鼠乳腺中的膳食脂肪。乳腺上皮细胞 增殖、凋亡和腺体发育正在研究中 在体内大鼠模型中。给予雌性Sprague-Dawley大鼠 致癌剂量方案的PhIP和放置在高脂肪或 低脂饮食。在致癌物给药后的不同时间， 对大鼠腺体进行癌前组织学检查， 改变。检测上皮细胞增殖和凋亡情况 免疫组织化学方法。乳房整体支架用于 检查致癌物质和饮食对发展的影响， 腺。初步结果表明，PhIP和膳食脂肪改变 细胞的末端芽结构中的增殖率 乳腺

To better understand the mechanisms involved in mammary gland cancer induction by the food-derived heterocyclic amine 2-amino-1-methyl-6- phenylimidazo[4,5-b]pyridine (PhIP) and the effect of dietary fat, studies are underway to assess the early alterations induced by PhIP and dietary fat in the rat mammary gland. Mammary gland epithelial cell proliferation, apoptosis, and glandular development are being studied in an in vivo rat model. Female Sprague-Dawley rats were administered a carcinogenic dose regimen of PhIP and placed on either a high fat or low fat diet. At various times after carcinogen administration, mammary glands from rats were examined for premalignant histological alterations. Epithelial cell proliferation and apoptosis were examined by immunohistochemical methods. Mammary whole mounts were used to examine the effect of carcinogen and diet on the development of the gland. The preliminary results indicate that PhIP and dietary fat alter the rate of proliferation in the terminal end bud structures of the mammary gland.