INNER HAIR CELL PHYSIOLOGY IN INTACT PERFUSED COCHLEAS

描述：这个项目试图了解声学反应是如何 哺乳动物听力器官的内毛细胞(IHC)的刺激是 受流经特定离子通道的离子电流的影响 毛细胞的基底膜。生物物理模型预测，在 电压激活离子通道的影响可能是 对毛细胞适应连续正弦的贡献 刺激和毛细胞相当于双色调抑制。全蜂窝 膜片钳记录技术将在完整的耳蜗中进行 从蒙古沙土鼠身上取出并通过灌流维持。毛细胞 生存能力将使用荧光染料进行评估，以表明 细胞内pH、膜电位和突触小泡循环。补片 钳位数据将被集成到生物物理毛细胞模型中。变化 在膜上，由突触小泡融合产生的电容将是 测量以表征发射机的释放。除了研究 毛细胞功能的基本生物物理学，这项工作也有助于 了解毛细胞的离子动态平衡，从而与 对代谢或其他创伤引起的听力损失的认识 毛细胞。

DESCRIPTION: This project seeks to understand how the responses to acoustic stimuli by inner hair cells (IHC) of the mammalian hearing organ are influenced by ionic currents passing through specific ion channels in the hair cell's basolateral membrane. A biophysical model predicts that among the effects of voltage-activated ion channels are likely to be a contribution to the adaptation of the hair cell to continuous sinusoidal stimulation and a hair cell equivalent of two-tone suppression. Whole-cell patch-clamp recording techniques will be carried out in intact cochleas removed from Mongolian gerbils and maintained by perfusion. Hair cell viability will be assessed using fluorescent dyes that indicate intracellular pH, membrane potential and synaptic vesicle recycling. Patch clamp data will be integrated into the biophysical hair cell model. Changes in membrane capacitance resulting from fusion of synaptic vesicles will be measured to characterize transmitter release. In addition to examining the basic biophysics of hair cell function, this work also contributes to the understanding of hair cell ionic homeostasis and thereby has relevance to understanding of hearing loss resulting from metabolic or other trauma to hair cells.