FUNCTION AND REGULATION OF THE CDC14 PROTEIN PHOSPHATASE

酿酒酵母的CDC14基因编码一种双特异性磷酸酶，其对于芽殖酵母中细胞周期的执行是必需的。 我们已经表明，Cdc14p的磷酸酶活性是细胞周期进程所必需的。 遗传学证据表明，Cdc14 p是退出有丝分裂所必需的，但Cdc14 p的底物和功能尚未确定。 我们的长期目标是确定Cdc14p在细胞周期调控中的重要作用，并确定其活性是如何调节的。 研究提出，以确定基板的Cdc14p和定义的机制（S）Cdc14p的活动是控制整个细胞周期。初步的研究结果已经确定了候选人的调节剂或磷酸化Cdc14p的蛋白质，这些将是我们的研究的一个主要重点。 这些信息将增加我们对退出有丝分裂所需的关键事件的理解。由于控制细胞周期的机制在整个进化过程中是高度保守的，因此这些在酵母中的研究对于理解人类细胞的增殖是如何控制的也将是有价值的。 编码调节细胞周期的蛋白质的基因是癌症突变的常见靶点。 破译控制细胞周期进程的调控途径可以提供有关这一过程如何在转化细胞中出错的重要信息，并可以确定阻断其生长的潜在靶点。

The CDC14 gene of Saccharomyces cerevisiae encodes a dual specificity phosphatase that is essential for the execution of the cell cycle in the budding yeast. We have shown that the phosphatase activity of Cdc14p is required for cell cycle progression. Genetic evidence suggests that Cdc14p is needed for exit from mitosis, but the substrates and function of Cdc14p have not been defined. Our long term goal is to identify the essential role that Cdc14p plays in cell cycle regulation and to determine how its activity is regulated. Studies are proposed to identify substrates for Cdcl4p and to define the mechanism(s) by which Cdc14p activity is controlled throughout the cell cycle. Preliminary findings have identified proteins that are candidates for regulators or phosphosubstrates of Cdc14p and these will be a major focus of our studies. This information will increase our understanding of the key events required for exit from mitosis. Because the mechanisms controlling the cell cycle are highly conserved throughout evolution, these studies in yeast will also be valuable in understanding how the proliferation of human cells is controlled. Genes encoding proteins that regulate the cell cycle are common targets for mutation in cancer. Deciphering the regulatory pathways that control cell cycle progression can provide important information about how this process goes awry in transformed cells and can identify potential targets for blocking their growth.