CEMENTOGENESIS--ROLE OF HERTWIGS EPITHELIAL ROOT SHEATH

牙周病和种植体的主要问题之一是 牙骨质、牙周等牙周组织的再生 韧带和牙槽骨。积累了大量的知识 然而，最近几年与骨再生相关的，我们对 牙骨质和牙周韧带的再生仍然不清楚。它是 这项研究的长期目标是了解细胞和 牙骨质形成和牙根附着的分子机制 使牙周韧带处于健康的牙周组织中。在此应用程序中， 我们专注于牙骨质的形成，特别是Hertwig‘s的作用 上皮根鞘(HERS)细胞参与这一过程。虽然这个角色 她的细胞在牙骨质形成中被广泛接受，准确地说 这些细胞的功能和命运仍然存在争议。我们建议 测试她的细胞合成和分泌牙釉质的假设- 形成中间和无细胞牙骨质的相关蛋白质，然后 这些细胞经过上皮-间充质转化为 成牙骨质细胞在牙根表面形成细胞牙骨质。这个项目 将利用永生鼠衍生的HERS细胞系 在我们的实验室建立。实验设计的目的是首先 确定她的细胞表达的釉质相关蛋白是否 体外牙骨质形成所必需的。监管机制主导 上皮间充质转化与牙骨质的性质 将对形成的细胞外基质进行研究。最后，我们会 检查使用她的细胞在体外确定的事件是否也 用小鼠动物模型置入体内。在此中生成的数据 研究将提供有价值的信息，与根的形成和 将有助于阐明HER在牙骨质形成中的作用以及可能的 如何提高牙缺失的诊断、治疗和预后 到衰老或牙周病。

One of the major problems in periodontal disease and tooth implants is the regeneration of periodontal tissues such as cementum, periodontal ligament and alveolar bone. Considerable knowledge as accumulated in recent years related to bone regeneration, however, our understanding of cementum and periodontal ligament regeneration is still obscure. It is the long term objective of this study to understand the cellular and molecular mechanisms underlying cementum formation and root attachment to periodontal ligament in the health periodontium. In this application, we focus on cementum formation and in particular the role of Hertwig's Epithelial Root Sheath (HERS) cells in this process. Although the role of HERS cells in cementum formation is widely accepted, the precise function and fate of these cells remains controversial. We propose to test the hypothesis that HERS cells synthesize and secrete enamel- related proteins which form intermediate and acellular cementum and then these cells undergo epithelial-mesenchymal transformation to become cementoblasts to form cellular cementum upon root surfaces. This project will take advantage of an Immortomouse-derived HERS cell line established in our laboratory. Experiments are designed to first determine if the enamel-related proteins expressed by the HERS cells are necessary for cementogenesis in vitro. The regulatory mechanisms leading to epithelial-mesenchymal transformation and the nature of the cementum extracellular matrix formed will be investigated. Finally, we will examine if the events determined using the HERS cells in vitro also take place in vivo using the mouse animal model. The data generated in this study will provide valuable information related to root formation and will help to clarify the role of HERS in cementum formation and possible ways to improve the diagnosis, treatment and prognosis of tooth loss due to aging or periodontal diseases.